DipA, a Pore-Forming Protein in the Outer Membrane of Lyme Disease Spirochetes Exhibits Specificity for the Permeation of Dicarboxylates 英文参考文献.docVIP

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DipA, a Pore-Forming Protein in the Outer Membrane of Lyme Disease Spirochetes Exhibits Specificity for the Permeation of Dicarboxylates 英文参考文献.doc

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DipA, a Pore-Forming Protein in the Outer Membrane of Lyme Disease Spirochetes Exhibits Specificity for the Permeation of Dicarboxylates 英文参考文献

DipA,aPore-FormingProteinintheOuterMembraneof LymeDiseaseSpirochetesExhibitsSpecificityforthe PermeationofDicarboxylates MarcusThein1,MariBonde2.,IgnasBunikis2.,KatrinDenker1,AlbertSickmann1,3,SvenBergstro¨m2, RolandBenz1,4 * 1Rudolf-Virchow-Center, DFG-Research Center for Experimental Biomedicine, University of Wu¨rzburg, Wu¨rzburg, Germany, 2Department of Molecular Biology, Umea? University,Umea?,Sweden,3DepartmentofBioanalytics,Leibniz-Institutfu¨rAnalytischeWissenschaften–ISAS,Dortmund,Germany,4SchoolofEngineeringandScience, JacobsUniversityBremen,Bremen,Germany Abstract LymediseaseBorreliaearehighlydependentontheuptakeofnutrientsprovidedbytheirhosts.Ourstudydescribesthe identificationofa36kDaproteinthatfunctionsasputativedicarboxylate-specificporinintheoutermembraneofLyme diseaseBorrelia.TheproteinwaspurifiedbyhydroxyapatitechromatographyfromBorreliaburgdorferiB31anddesignated asDipA,fordicarboxylate-specificporinA.DipAwaspartiallysequenced,andcorrespondinggeneswereidentifiedinthe genomesofB.burgdorferiB31,BorreliagariniiPBiandBorreliaafzeliiPKo.DipAexhibitshighhomologytotheOms38porins ofrelapsingfeverBorreliae.B.burgdorferiDipAwascharacterizedusingtheblacklipidbilayerassay.Theproteinhasasingle- channelconductanceof50pSin1MKCl,isslightlyselectiveforanionswithapermeabilityratioforcationsoveranionsof 0.57 in KCl and is not voltage-dependent. The channel could be partly blocked by different di- and tricarboxylic anions. Particularhighstabilityconstantsuptoabout28,000l/mol(in0.1MKCl)wereobtainedamongthe11testedanionsfor oxaloacetate,2-oxoglutarateandcitrate.TheresultsimplythatDipAformsaporinspecificfordicarboxylateswhichmay playanimportantrolefortheuptakeofspecificnutrientsindifferentBorreliaspecies. Citation:TheinM,BondeM,BunikisI,DenkerK,SickmannA,etal.(2012)DipA,aPore-FormingProteinintheOuterMembraneofLymeDiseaseSpirochetes ExhibitsSpecificityforthePermeationofDicarboxylates.PLoSONE7(5):e36523.doi:10.1371/journal.pone.0036523 Editor:MichelR.Popoff,InstitutePas