Discovery and Analysis of Evolutionarily Conserved Intronic Splicing Regulatory Elements 英文参考文献.docVIP

下载本文档

3
0
约7.97万字
约 16页
2017-05-11 发布于上海
举报
版权申诉

Discovery and Analysis of Evolutionarily Conserved Intronic Splicing Regulatory Elements 英文参考文献.doc

1、本文档共16页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Discovery and Analysis of Evolutionarily Conserved Intronic Splicing Regulatory Elements 英文参考文献

DiscoveryandAnalysisofEvolutionarily ConservedIntronicSplicingRegulatory Elements Gene W. Yeo1,2*,Eric L.Van Nostrand1,2¤,Tiffany Y.Liang1,2 1Crick-JacobsCenterforTheoreticalandComputationalBiology,SalkInstitute,LaJolla,California,UnitedStatesofAmerica,2LaboratoryofGenetics,SalkInstitute,LaJolla, California,UnitedStatesofAmerica Knowledgeof thefunctional cis-regulatory elements that regulateconstitutive andalternative pre-mRNA splicing is fundamental for biology and medicine. Here we undertook a genome-wide comparative genomics approach using available mammalian genomes to identify conserved intronic splicing regulatory elements (ISREs). Our approach yielded314ISREs,andinsertionsof;70ISREsbetweencompetingsplicesitesdemonstratedthat84%ofISREsaltered 59 and 94% altered 39 splice site choice in human cells. Consistent with our experiments, comparisons of ISREs to knownsplicingregulatoryelementsrevealedthat40%–45%ofISREsmighthavedualrolesasexonicsplicingsilencers. SupportingaroleforISREsinalternativesplicing,wefoundthat30%–50%ofISREswereenrichednearalternatively spliced(AS)exons,andincludedalmostallknownbindingsitesoftissue-specificalternativesplicingfactors.Further, weobservedthatgenesharboringISRE-proximalexonshavebiasesfortissueexpressionandmolecularfunctionsthat are ISRE-specific. Finally, we discovered that for Nova1, neuronal PTB, hnRNP C, and FOX1, the most frequently occurringISREproximaltoanalternativeconservedexoninthesplicingfactorstronglyresembleditsownknownRNA bindingsite,suggestinganovelapplicationofISREdensityandthepropensityforsplicingfactorstoauto-regulateto associate RNA binding sites to splicing factors. Our results demonstrate that ISREs are crucial building blocks in understandinggeneralandtissue-specificASregulationandthebiologicalpathwaysandfunctionsregulatedbythese ASevents. Citation:YeoGW,VanNostrandEL,LiangTY(2007)Discoveryandanalysisofevolutionarilyconservedintronicsplicingregulatoryelements.PLoSGenet3(5):e85.doi:10. 1371/journal.pgen.003008