Dissecting Phaseolus vulgaris Innate Immune System against Colletotrichum lindemuthianum Infection 英文参考文献.docVIP
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Dissecting Phaseolus vulgaris Innate Immune System against Colletotrichum lindemuthianum Infection 英文参考文献
DissectingPhaseolusvulgarisInnateImmuneSystem
againstColletotrichumlindemuthianumInfection
PaulaRodriguesOblessuc1.,AlineBorges2.,BabluChowdhury1,DanielleGrego′rioGomesCaldas2 ,Siu
MuiTsai2,LuisEduardoAranhaCamargo3,MaeliMelotto1*
1DepartmentofBiology,UniversityofTexas,Arlington,Texas,UnitedStatesofAmerica,2CENA,UniversidadedeSa?o Paulo,Piracicaba,SP,Brazil,3Departamentode
Fitopatologia,ESALQ,UniversidadedeSa?o Paulo,Piracicaba,SP,Brazil
Abstract
Background:ThegenusColletotrichumisoneofthemosteconomicallyimportantplantpathogens,causinganthracnoseon
awiderangeofcropsincludingcommonbeans(PhaseolusvulgarisL.).Cropyieldcanbedramaticallydecreaseddepending
ontheplantcultivarusedandtheenvironmentalconditions.Thisstudyaimedtoidentifypotentialgeneticcomponentsof
thebeanimmunesystemtoprovideenvironmentallyfriendlycontrolmeasuresagainstthisfungus.
MethodologyandPrincipalFindings:Asthecommonbeanisnotamenabletoreversegeneticstoexplorefunctionality
and its genome is not fully curated, we used putative Arabidopsis orthologs of bean expressed sequence tag (EST) to
performbioinformaticanalysisandexperimentalvalidationofgeneexpressiontoidentifycommonbeangenesregulated
duringtheincompatibleinteractionwithC.lindemuthianum.Similartomodelpathosystems,GeneOntology(GO)analysis
indicated that hormone biosynthesis and signaling in common beans seem to be modulated by fungus infection. For
instance,cytokininandethyleneresponseswereup-regulatedandjasmonicacid,gibberellin,andabscisicacidresponses
weredown-regulated,indicatingthatthesehormonesmayplayacentralroleinthispathosystem.Importantly,wehave
identifiedputativebeangeneorthologsofArabidopsisgenesinvolvedintheplantimmunesystem.Basedonexperimental
validationofgeneexpression,weproposethathypersensitivereactionaspartofeffector-triggeredimmunitymayoperate,
at least in part, by down-regulating genes, such as FLS2-like and MKK5-like, putative orthologs of the Arabidopsis genes
involvedinpathogenperceptionanddownstreamsignaling.
Conclus
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