Down Regulation of a Gene for Cadherin, but Not Alkaline Phosphatase, Associated with Cry1Ab Resistance in the Sugarcane Borer Diatraea saccharalis 英文参考文献.docVIP

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Down Regulation of a Gene for Cadherin, but Not Alkaline Phosphatase, Associated with Cry1Ab Resistance in the Sugarcane Borer Diatraea saccharalis 英文参考文献.doc

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Down Regulation of a Gene for Cadherin, but Not Alkaline Phosphatase, Associated with Cry1Ab Resistance in the Sugarcane Borer Diatraea saccharalis 英文参考文献

DownRegulationofaGeneforCadherin,butNot AlkalinePhosphatase,AssociatedwithCry1Ab ResistanceintheSugarcaneBorerDiatraeasaccharalis YunlongYang1,YuChengZhu2*,JamesOttea1,ClaudiaHusseneder1,B.RogersLeonard1,CraigAbel2,3, RandallLuttrell2,FangnengHuang1 1DepartmentofEntomology,LouisianaStateUniversityAgriculturalCenter,BatonRouge,Louisiana,UnitedStatesofAmerica,2SouthernInsectManagementResearch Unit,AgriculturalResearchService,UnitedStatesDepartmentofAgriculture,Stoneville,Mississippi,UnitedStatesofAmerica,3CornInsectsandCropGeneticsResearch Unit,AgriculturalResearchService,UnitedStatesDepartmentofAgriculture,Ames,Iowa,UnitedStatesofAmerica Abstract The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins(i.e.,Cry1Ab)inSouthAmericaandthemid-southernregionoftheUnitedStates.Evolutionofinsecticideresistance in such target pests is a major threat to the durability of transgenic Bt crops. Understanding the pests’ resistance mechanisms will facilitate development of effective strategies for delaying or countering resistance. Alterations in expressionofcadherin-andalkalinephosphatase(ALP)havebeenassociatedwithBtresistanceinseveralspeciesofpest insects.Inthisstudy,neithertheactivitynorgeneregulationofALPwasassociatedwithCry1AbresistanceinD.saccharalis. TotalALPenzymaticactivitywassimilarbetweenCry1Ab-susceptible (Cry1Ab-SS)and-resistant(Cry1Ab-RR)strainsofD. saccharalis. In addition, expression levels of three ALP genes were also similar between Cry1Ab-SS and -RR, and cDNA sequences did not differ between susceptible and resistant larvae. In contrast, altered expression of a midgut cadherin (DsCAD1)wasassociatedwiththeCry1Abresistance.WhereascDNAsequencesofDsCAD1wereidenticalbetweenthetwo strains,thetranscriptabundanceofDsCAD1wassignificantlylowerinCry1Ab-RR.ToverifytheinvolvementofDsCAD1in susceptibility to Cry1Ab, RNA interference (RNAi) was employed to knock-down DsCAD1 expression in the susce