Dual Role for the O-Acetyltransferase OatA in Peptidoglycan Modification and Control of Cell Septation in Lactobacillus plantarum 英文参考文献.docVIP

DualRolefortheO-AcetyltransferaseOatAin PeptidoglycanModificationandControlofCellSeptation inLactobacillusplantarum ElvisBernard1,2,3,ThomasRolain3,BlandineDavid3,GuillaumeAndre′4,VincentDupres4, YvesF.Dufre?ne4,BernardHallet3,Marie-PierreChapot-Chartier1,2,PascalHols3* 1InstitutNationaldelaRechercheAgronomique,UMR1319Micalis,Jouy-en-Josas,France,2AgroParisTech,UMRMicalis,Jouy-en-Josas,France,3BiochimieetGe′ne′tique Mole′culaireBacte′rienne,InstitutdesSciencesdelaVie,Universite′catholiquedeLouvain,Louvain-la-Neuve,Belgium,4BioandSoftMatter,InstituteofCondensedMatter andNanosciences,Universite′ catholiquedeLouvain,Louvain-la-Neuve,Belgium Abstract Untilnow,peptidoglycanO-acetyltransferases(Oat)wereonlydescribedfortheirpeptidoglycanO-acetylatingactivityand for their implication in the control of peptidoglycan hydrolases. In this study, we show that a Lactobacillus plantarum mutant lacking OatA is unable to uncouple cell elongation and septation. Wild-type cells showed an elongation arrest duringseptationwhileoatAmutantcellscontinuedtoelongateataconstantratewithoutanyobservablepauseduringthe celldivisionprocess.Remarkably,thisdefectdoesnotresultfromadefaultinpeptidoglycanO-acetylation,sinceitcanbe rescued by wild-type OatA as well as by a catalytic mutant or a truncated variant containing only the transmembrane domainoftheprotein.Consistentwithapotentialinvolvementindivision,OatApreferentiallylocalizesatmid-cellbefore membrane invagination and remains at this position until the end of septation. Overexpression of oatA or its inactive variantsinducesseptation-specificaberrations,includingasymmetricalanddualseptumformation.Overproductionofthe divisioninhibitors,MinCorMinD,leadstocellfilamentationinthewildtypewhilecurvedandbranchedcellsareobserved in the oatA mutant, suggesting that the Min system acts differently on the division process in the absence of OatA. Altogether, the results suggest that OatA plays a key role in the spatio-temporal control of septation, irres