Drosophila Cuticular Hydrocarbons Revisited Mating Status Alters Cuticular Profiles 英文参考文献.docVIP

DrosophilaCuticularHydrocarbonsRevisited:Mating StatusAltersCuticularProfiles ClaudeEveraerts1*.,Jean-PierreFarine1.,MatthewCobb2,Jean-Franc?oisFerveur1 1Centre des Sciences du Gou?t et de l’Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Universite′ de Bourgogne, Agrosup, Dijon, France, 2Faculty of Life Sciences, UniversityofManchester,Manchester,UnitedKingdom Abstract Most living organisms use pheromones for inter-individual communication. In Drosophila melanogaster flies, several pheromones perceived either by contact/at a short distance (cuticular hydrocarbons, CHs), or at a longer distance (cis- vaccenylacetate,cVA),affectcourtshipandmatingbehaviours.However,ithasnotpreviouslybeenpossibletoprecisely identifyallpotentialpheromonalcompoundsandsimultaneouslymonitortheirvariationonatimescale.Toovercomethis limitation, we combined Solid Phase Micro-Extraction with gas-chromatography coupled with mass-spectrometry. This allowed us (i) to identify 59 cuticular compounds, including 17 new CHs; (ii) to precisely quantify the amount of each compoundthatcouldbedetectedbyanotherfly,and(iii)tomeasurethevariationofthesesubstancesasafunctionof agingandmating.Sex-specificvariationappearedwithage,whilematingaffectedcuticularcompoundsinbothsexeswith three possible patterns: variation was (i) reciprocal in the two sexes, suggesting a passive mechanical transfer during mating,(ii)parallelinbothsexes,suchasforcVAwhichstrikinglyappearedduringmating,or(iii)unilateral,presumablyasa result of sexual interaction. We provide a complete reassessment of all Drosophila CHs and suggest that the chemical conversationbetweenmaleandfemalefliesisfarmorecomplexthanisgenerallyaccepted.Weconcludethatfocusingon individual compounds will not provide a satisfactory understanding of the evolution and function of chemical communicationinDrosophila. Citation:EveraertsC,FarineJ-P,CobbM,FerveurJ-F(2010)DrosophilaCuticularHydrocarbonsRevisited:MatingStatusAltersCuticularProfiles.PLoSONE5(3): e9607.doi:10.137