Dynamics of Rad9 Chromatin Binding and Checkpoint Function Are Mediated by Its Dimerization and Are Cell Cycle–Regulated by CDK1 Activity 英文参考文献.docVIP

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Dynamics of Rad9 Chromatin Binding and Checkpoint Function Are Mediated by Its Dimerization and Are Cell Cycle–Regulated by CDK1 Activity 英文参考文献.doc

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Dynamics of Rad9 Chromatin Binding and Checkpoint Function Are Mediated by Its Dimerization and Are Cell Cycle–Regulated by CDK1 Activity 英文参考文献

DynamicsofRad9ChromatinBindingandCheckpoint FunctionAreMediatedbyItsDimerizationandAreCell Cycle–RegulatedbyCDK1Activity MagdaGranata1.,FedericoLazzaro1.,DanieleNovarina1,DavidePanigada1,FabioPuddu1 ,Carla ManuelaAbreu2,RameshKumar2,MurielGrenon2,NoelF.Lowndes2,PaoloPlevani1* ,Marco Muzi-Falconi1* 1Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita` degli Studi di Milano, Milano, Italy, 2Centre for Chromosome Biology, School of Natural Science, NationalUniversityofIrelandGalway,Galway,Ireland Abstract Saccharomycescerevisiae Rad9isrequiredforaneffectiveDNAdamageresponsethroughoutthecellcycle.Assembly of Rad9onchromatinafterDNAdamageispromotedbyhistonemodificationsthatcreatedockingsitesforRad9recruitment, allowing checkpoint activation. Rad53 phosphorylation is also dependent upon BRCT-directed Rad9 oligomerization; however,thecrosstalkbetweenthesemoleculardeterminantsandtheirfunctionalsignificancearepoorlyunderstood.Here wereportthat,intheG1andMphasesofthecellcycle,bothconstitutiveandDNAdamage-dependentRad9chromatin associationrequireitsBRCTdomains.InG1cells,GSTorFKBPdimerizationmotifscansubstitutetotheBRCTdomainsfor Rad9 chromatin binding and checkpoint function. Conversely, forced Rad9 dimerization in M phase fails to promote its recruitmentontoDNA,althoughitsupportsRad9checkpointfunction.Infact,aparallelpathway,independentonhistone modifications and governed by CDK1 activity, allows checkpoint activation in the absence of Rad9 chromatin binding. CDK1-dependentphosphorylationofRad9onSer11leadstospecificinteractionwithDpb11,allowingRad53activationand bypassingtherequirementforthehistonebranch. Citation:GranataM,LazzaroF,NovarinaD,PanigadaD,PudduF,etal.(2010)DynamicsofRad9ChromatinBindingandCheckpointFunctionAreMediatedbyIts DimerizationandAreCellCycle–RegulatedbyCDK1Activity.PLoSGenet6(8):e1001047.doi:10.1371/journal.pgen.1001047 Editor:GregoryP.Copenhaver,TheUniversityofNorthCarolinaatChapelHill,UnitedStatesofAmerica ReceivedDecember24,2009