Dynamic Alteration of the Colonic Microbiota in Intestinal Ischemia-Reperfusion Injury 英文参考文献.docVIP

下载本文档

5
0
约4.11万字
约 9页
2017-05-11 发布于上海
举报
版权申诉

Dynamic Alteration of the Colonic Microbiota in Intestinal Ischemia-Reperfusion Injury 英文参考文献.doc

1、本文档共9页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Dynamic Alteration of the Colonic Microbiota in Intestinal Ischemia-Reperfusion Injury 英文参考文献

DynamicAlterationoftheColonicMicrobiotain IntestinalIschemia-ReperfusionInjury FanWang,QiurongLi,ChenyangWang,ChunTang,JieshouLi* ResearchInstituteofGeneralSurgery,JinlingHospital,SchoolofMedicine,NanjingUniversity,Nanjing,China Abstract Background: Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesisoftheinjury.Thisstudyisaimedatunravelingcolonicmicrobiotaalterationpatternandidentifyingspecific bacterialspeciesthatdiffersignificantlyaswellasobservingcolonicepitheliumchangeinthesameinjurymodelduringthe reperfusiontimecourse. Methodology/Principal Findings: Denaturing gradient gel electrophoresis (DGGE) was used to monitor the colonic microbiotaofcontrolratsandexperimentalratsthatunderwent0.5hourischemiaand1,3,6,12,24,and72hoursfollowing reperfusion respectively. The microbiota similarity, bacterial diversity and species that characterized the dysbiosis were estimatedbasedontheDGGEprofilesusingacombinationofstatisticalapproaches.Theinterestedbacterialspeciesinthe gelwerecutandsequencedandweresubsequentlyquantifiedandconfirmedwithreal-timePCR.Meanwhile,theepithelial barrierwascheckedbymicroscopyandD-lactateanalysis.Colonicflorachangedearlyanddifferedsignificantlyat6hours afterreperfusionandthenstartedtorecover.TheshiftswerecharacterizedbytheincreaseofEscherichiacoliandPrevotella oralis,andLactobacilliproliferationtogetherwithepitheliahealing. Conclusion/Significance: This study shows for the first time that intestinal ischemia-reperfusion results in colonic flora dysbiosisthatfollowsepitheliadamage,andidentifiesthebacterialspeciesthatcontributemost. Citation:WangF,LiQ,WangC,TangC,LiJ(2012)DynamicAlterationoftheColonicMicrobiotainIntestinalIschemia-ReperfusionInjury.PLoSONE7(7):e42027. doi:10.1371/journal.pone.0042027 Editor:MarkusM.Heimesaat,Charite′,CampusBenjaminFranklin,Germany ReceivedApril13,2012;AcceptedJune29,2012;PublishedJuly27,2012 Copyright: ? 2012 Wang et al. This is an open-access