Duplication and Evolution of devA-Like Genes in Streptomyces Has Resulted in Distinct Developmental Roles 英文参考文献.docVIP

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Duplication and Evolution of devA-Like Genes in Streptomyces Has Resulted in Distinct Developmental Roles 英文参考文献.doc

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Duplication and Evolution of devA-Like Genes in Streptomyces Has Resulted in Distinct Developmental Roles 英文参考文献

DuplicationandEvolutionofdevA-LikeGenesin StreptomycesHasResultedinDistinctDevelopmental Roles LauraC.Clark,PaulA.Hoskisson* StrathclydeInstituteofPharmacyandBiomedicalScience,UniversityofStrathclyde,Glasgow,UnitedKingdom Abstract Understandingmorphologicaltransformationsisessentialtoelucidatingtheevolutionanddevelopmentalbiologyofmany organisms.TheGram-positivesoilbacterium,Streptomycescoelicolorhasacomplexlifecyclewhichlendsitselfwelltosuch studies.Werecentlyidentifiedatranscriptionalregulator,devA,whichisrequiredforcorrectsporulationinthisorganism, withmutantsformingshort,mis-septateaerialhyphae.devAishighlyconservedwithintheStreptomycesgenusalongwitha duplicatecopy,devE.DisruptionofdevEindicatesthisgenealsoplaysaroleinsporulation;howeverthephenotypeofa devEmutantdiffersfromadevAmutant,forminglongun-septateaerialhyphae.TranscriptionalanalysisofdevAanddevE indicates that they are expressed at different stages of the lifecycle. This suggests that following duplication they have divergedinregulationandfunction.AnalysisoffullysequencedactinomycetegenomesshowsthatdevAisfoundinasingle copy in morphologically simpler actinobacteria, suggesting that duplication has lead to increased morphological complexity. Complementation studies with devA from Salinispora, which sporulates but does not form aerial hyphae, indicatestheancestralgenecannotcomplementdevAordevE,suggestingneo-functionalisationhasoccurred.Analysisof the synonymous andnon-synonymous nucleotide changes within the devA paralogues suggest subfunctionalisation has occurredasbothcopieshavedivergedfromtheancestralsequences.Divergenceisalsoasymmetricwithahigherlevelof functionalconstraint observedintheDNAbindingdomaincomparedwiththeeffectorbinding/oligomerisation domain, suggestingdiversificationinthesubstratespecificityoftheseparalogueshascontributedtotheirevolution. Citation: Clark LC, Hoskisson PA (2011) Duplication and Evolution of devA-Like Genes in Streptomyces Has Resulted in Distinct Developmental R