Early Interferon-γ Production in Human Lymphocyte Subsets in Response to Nontyphoidal Salmonella Demonstrates Inherent Capacity in Innate Cells 英文参考文献.docVIP

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Early Interferon-γ Production in Human Lymphocyte Subsets in Response to Nontyphoidal Salmonella Demonstrates Inherent Capacity in Innate Cells 英文参考文献.doc

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Early Interferon-γ Production in Human Lymphocyte Subsets in Response to Nontyphoidal Salmonella Demonstrates Inherent Capacity in Innate Cells 英文参考文献

EarlyInterferon-cProductioninHumanLymphocyte SubsetsinResponsetoNontyphoidalSalmonella DemonstratesInherentCapacityinInnateCells TonneyS.Nyirenda1,2.,AnnaE.Seeley1.,WilsonL.Mandala2,3,MarkT.Drayson1,CalmanA. MacLennan1,4 * 1MedicalResearchCouncilCentreforImmuneRegulationandClinicalImmunologyService,InstituteofBiomedicalResearch,SchoolofImmunityandInfection,Collegeof Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom, 2Malawi-Liverpool-Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre, Malawi, 3Department of Biochemistry, College of Medicine, University of Malawi, Blantyre, Malawi, 4Division of Medical Microbiology,SchoolofInfectionandHostDefence,UniversityofLiverpool,Liverpool,UnitedKingdom Abstract Background:NontyphoidalSalmonellaefrequentlycauselife-threateningbacteremiainsub-SaharanAfrica.Youngchildren andHIV-infectedadultsareparticularlysusceptible.Highcase-fatalityratesandincreasingantibioticresistancerequirenew approachestothemanagementofthisdisease.ImpairedcellularimmunitycausedbydefectsintheThelper1pathway lead to intracellular disease with Salmonella that can be countered by IFNc administration. This report identifies the lymphocytesubsetsthatproduceIFNcearlyinSalmonellainfection. Methodology: Intracellular cytokine staining was used to identify IFNc production in blood lymphocyte subsets of ten healthyadultswithantibodiestoSalmonella(asevidenceofimmunitytoSalmonella),inresponsetostimulationwithlive and heat-killed preparations of the D23580 invasive African isolate of Salmonella Typhimurium. The absolute number of IFNc-producingcellsininnate,innate-likeandadaptivelymphocytesubpopulationswasdetermined. PrincipalFindings:EarlyIFNcproductionwasfoundintheinnate/innate-likelymphocytesubsets:cd-Tcells,NKcellsand NK-likeTcells.SignificantlyhigherpercentagesofsuchcellsproducedIFNccomparedtoadaptiveab-Tcells(Student’st + + test,P,0.001and#0.02foreachinnatesubsetcompared,respectively,wi