Echinococcus granulosus Antigen B Structure Subunit Composition and Oligomeric States 英文参考文献.docVIP

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Echinococcus granulosus Antigen B Structure Subunit Composition and Oligomeric States 英文参考文献.doc

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Echinococcus granulosus Antigen B Structure Subunit Composition and Oligomeric States 英文参考文献

EchinococcusgranulosusAntigenBStructure:Subunit CompositionandOligomericStates KarinaM.Monteiro1,MateusB.Cardoso2,CristianFollmer3,Na′dyaP.daSilveira4,DaianiM.Vargas1, ElliotW.Kitajima5,ArnaldoZaha1,HenriqueB.Ferreira1* 1Laborato′riodeBiologiaMoleculardeCesto′deosandLaborato′riodeGeno?micaEstruturaleFuncional,CentrodeBiotecnologia,UniversidadeFederaldoRioGrandedo Sul,PortoAlegre,RioGrandedoSul,Brazil,2Laborato′rioNacionaldeLuzS?′ncrotron(LNLS),Campinas,Sa?o Paulo,Brazil,3DepartamentodeF?′sico-Qu?′mica,Institutode Qu?′mica,UniversidadeFederaldoRiodeJaneiro,RiodeJaneiro,RiodeJaneiro,Brazil,4InstitutodeQu?′mica,UniversidadeFederaldoRioGrandedoSul,PortoAlegre,Rio GrandedoSul,Brazil,5DepartamentodeEntomologia,FitopatologiaeZoologiaAgr?′cola,EscolaSuperiordeAgriculturaLuizdeQueiroz(ESALQ),UniversidadedeSa?o Paulo,Piracicaba,Sa?o Paulo,Brazil Abstract Background:AntigenB(AgB)isthemajorproteinsecretedbytheEchinococcusgranulosusmetacestodeandisinvolvedin key host-parasite interactions during infection. The full comprehension of AgB functions depends on the elucidation of severalstructuralaspectsthatremainunknown,suchasitssubunitcompositionandoligomericstates. Methodology/Principal Findings: The subunit composition of E. granulosus AgB oligomers from individual bovine and human cysts was assessed by mass spectrometry associated with electrophoretic analysis. AgB8/1, AgB8/2, AgB8/3 and AgB8/4subunitswereidentifiedinallsamplesanalyzed,andanAgB8/2variant(AgB8/2v8)wasfoundinonebovinesample. The exponentially modified protein abundance index (emPAI) was used to estimate the relative abundance of the AgB subunits,revealingthatAgB8/1subunitwasrelativelyoverrepresentedinallsamples.TheabundanceofAgB8/3subunit varied between bovine and human cysts. The oligomeric states formed by E. granulosus AgB and recombinant subunits available,rAgB8/1,rAgB8/2andrAgB8/3,werecharacterizedbynativePAGE,lightscatteringandmicroscopy.Recombinant subunitsshowedmarkedlydistinctoligomerizationbeha