Effects of Sodium Azide on the Abundance of Prokaryotes and Viruses in Marine Samples 英文参考文献.docVIP

EffectsofSodiumAzideontheAbundanceof ProkaryotesandVirusesinMarineSamples Christian Winter1*, Marie-Emmanuelle Kerros2,3, Markus G. Weinbauer2,3 1 Department of Marine Biology, University of Vienna, Vienna, Austria, 2 Microbial Ecology and Biogeochemistry Group, Centre National de la Recherche Scientifique, Laboratoire d’Oce′anographie de Villefranche, Villefranche-sur-Mer, France 3 Laboratoire d’Oce′anographie de Villefranche, Universite P′ierre et Marie Curie-Paris 6, Villefranche-sur-Mer, France Abstract Flow cytometry is set to become the standard method for enumerating prokaryotes and viruses in marine samples. However, the samples need to be flash-frozen in liquid nitrogen directly after aldehyde fixation. Because liquid nitrogen may not always be available, we tested the potential of sodium azide as a preservative for prokaryotes and viruses in marine samples as a possible alternative. For that we conducted incubation experiments with untreated and sodium azide treated marine water samples at 4uC and room temperature. The data indicate that sodium azide cannot be used to maintain marine samples used for the enumeration of prokaryotes and viruses. Citation: Winter C, Kerros M-E, Weinbauer MG (2012) Effects of Sodium Azide on the Abundance of Prokaryotes and Viruses in Marine Samples. PLoS ONE 7(5): e37597. doi:10.1371/journal.pone.0037597 Editor: Bryan A. White, University of Illinois, United States of America Received March 21, 2012; Accepted April 25, 2012; Published May 18, 2012 Copyright: ? 2012 Winter et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: Financial support was provided by the