Elevated Non-Esterified Fatty Acid Concentrations during Bovine Oocyte Maturation Compromise Early Embryo Physiology 英文参考文献.docVIP

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Elevated Non-Esterified Fatty Acid Concentrations during Bovine Oocyte Maturation Compromise Early Embryo Physiology 英文参考文献.doc

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Elevated Non-Esterified Fatty Acid Concentrations during Bovine Oocyte Maturation Compromise Early Embryo Physiology 英文参考文献

ElevatedNon-EsterifiedFattyAcidConcentrationsduring BovineOocyteMaturationCompromiseEarlyEmbryo Physiology VeerleVanHoeck1*,RogerG.Sturmey2,PabloBermejo-Alvarez3,DimitriosRizos3,AlfonsoGutierrez- Adan3,HenryJ.Leese2,PeterE.J.Bols1,JoL.M.R.Leroy1 1LaboratoryforVeterinaryPhysiologyandBiochemistry,DepartmentofVeterinarySciences,FacultyofBiomedical,PharmaceuticalandVeterinarySciences,Universityof Antwerp,Wilrijk,Belgium,2Hull-YorkMedicalSchool,UniversityofHull,Hull,UnitedKingdom, 3DepartamentodeReproduccio′nAnimalyConservacio′ndeRecursos Zoogene′ticos,INIA,Madrid,Spain Abstract Elevatedconcentrationsofserumnon-esterifiedfattyacids(NEFA),associatedwithmaternaldisorderssuchasobesityand type II diabetes, alter the ovarian follicular micro-environment and have been associated with subfertility arising from reduced oocyte developmental competence. We have asked whether elevated NEFA concentrations during oocyte maturationaffectthedevelopmentandphysiologyofzygotesformedfromsuchoocytes,usingthecowasamodel.The zygotesweregrowntoblastocysts,whichwereevaluatedfortheirqualityintermsofcellnumber,apoptosis,expressionof keygenes,aminoacidturnoverandoxidativemetabolism.OocytematurationunderelevatedNEFAconcentrationsresulted inblastocystswithsignificantlylowercellnumber,increasedapoptoticcellratioandalteredmRNAabundanceofDNMT3A, IGF2RandSLC2A1.Inaddition,theblastocystsdisplayedreducedoxygen,pyruvateandglucoseconsumption,up-regulated lactateconsumptionandhigheraminoacidmetabolism.Thesedataindicatethatexposureofmaturingoocytestoelevated NEFAconcentrationshasanegativeimpactonfertilitynotonlythroughareductioninoocytedevelopmentalcapacitybut throughcompromisedearlyembryoquality,viabilityandmetabolism. Citation:VanHoeckV,SturmeyRG,Bermejo-AlvarezP,RizosD,Gutierrez-AdanA,etal.(2011)ElevatedNon-EsterifiedFattyAcidConcentrationsduringBovine OocyteMaturationCompromiseEarlyEmbryoPhysiology.PLoSONE6(8):e23183.doi:10.1371/journal.pone.0023183 Editor:SamuelKim,UniversityofKansasMedicalCenter