Elevated Incidence of Dental Caries in a Mouse Model of Cystic Fibrosis 英文参考文献.docVIP

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Elevated Incidence of Dental Caries in a Mouse Model of Cystic Fibrosis 英文参考文献.doc

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Elevated Incidence of Dental Caries in a Mouse Model of Cystic Fibrosis 英文参考文献

ElevatedIncidenceofDentalCariesinaMouseModelof CysticFibrosis MarceloA.Catala′n1¤,KathleenScott-Anne2,MarliseI.Klein2,HyunKoo2,WilliamH.Bowen2,JamesE. Melvin1*¤ 1Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York, United States of America, 2Center for Oral Biology, UniversityofRochesterMedicalCenter,Rochester,NewYork,UnitedStatesofAmerica Abstract Background:Dentalcariesisthesinglemostprevalentandcostlyinfectiousdiseaseworldwide,affectingmorethan90%of the population in the U.S. The development of dental cavities requires the colonization of the tooth surface by acid- producing bacteria, such as Streptococcus mutans. Saliva bicarbonate constitutes the main buffering system which neutralizesthepHfallgeneratedbytheplaquebacteriaduringsugarmetabolism.WefoundthatthesalivapHisseverely decreased in a mouse model of cystic fibrosis disease (CF). Given the close relationship between pH and caries development,wehypothesizedthatcariesincidencemightbeelevatedinthemouseCFmodel. Methodology/PrincipalFindings:WeinducedcariouslesionsinCFandwildtypemicebyinfectingtheiroralcavitywithS. mutans,awell-studiedcariogenicbacterium.Afterinfection,themicewerefedahigh-sucrosedietfor5weeks(diet2000).The miceweretheneuthanizedandtheirjawsremovedforcariesscoringandbacterialcounting.Adramaticincreaseincariesand severityoflesionsscoreswereapparentinCFmicecomparedtotheirwildtypelittermates.Theelevatedincidenceofcarious lesions correlated with a striking increase in the S. mutans viable population in dental plaque (20-fold increase in CF vs. wildtypemice;pvalue,0.003;ttest).Wealsofoundthatthepilocarpine-stimulatedsalivabicarbonateconcentrationwas significantlyreducedinCFmice(1662mMvs.3162mM,CFandwildtypemice,respectively;pvalue,0.01;ttest). Conclusions/Significance: Considering that bicarbonate is the most important pH buffering system in saliva, and the adherenceandsurvivalofaciduricbacteriasuchasS.mutansareenhancedatlowpHvalues,wespeculatethatt