Endosperm Tolerance of Paternal Aneuploidy Allows Radiation Hybrid Mapping of the Wheat D-Genome and a Measure of γ Ray-Induced Chromosome Breaks 英文参考文献.docVIP

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Endosperm Tolerance of Paternal Aneuploidy Allows Radiation Hybrid Mapping of the Wheat D-Genome and a Measure of γ Ray-Induced Chromosome Breaks 英文参考文献.doc

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Endosperm Tolerance of Paternal Aneuploidy Allows Radiation Hybrid Mapping of the Wheat D-Genome and a Measure of γ Ray-Induced Chromosome Breaks 英文参考文献

EndospermToleranceofPaternalAneuploidyAllows RadiationHybridMappingoftheWheatD-Genomeand aMeasureofcRay-InducedChromosomeBreaks VijayK.Tiwari1,OscarRiera-Lizarazu2,HilaryL.Gunn1,KaSandraLopez1,M.JavedIqbal3, ShahryarF.Kianian3,JeffreyM.Leonard1* 1DepartmentofCropandSoilScience,OregonStateUniversity,Corvallis,Oregon,UnitedStatesofAmerica,2InternationalCropsResearchInstitutefortheSemi-Arid Tropics(ICRISAT),Patancheru,AndhraPradesh,India,3DepartmentofPlantSciences,NorthDakotaStateUniversity,Fargo,NorthDakota,UnitedStatesofAmerica Abstract Physical mapping and genome sequencing are underway for the 17Gb wheat genome. Physical mapping methods independentofmeioticrecombination,suchasradiationhybrid(RH)mapping,willaidpreciseanchoringofBACcontigsin the large regions of suppressed recombination in Triticeae genomes. Reports of endosperm development following pollination with irradiated pollen at dosages that cause embryo abortion prompted us to investigate endosperm as apotentialsourceofRHmappinggermplasm.Here,wereportanovelapproachtoconstructRHbasedphysicalmapsofall seven D-genome chromosomes of the hexaploid wheat ‘Chinese Spring’, simultaneously. An 81-member subset of endospermsamplesderivedfrom20-Gyirradiatedpollenwasgenotypedfordeletions,and737markersweremappedon seven D-genome chromosomes. Analysis of well-defined regions of six chromosomes suggested a map resolution of ,830kbcouldbeachieved;thisestimatewasvalidatedwithassaysofmarkersfromasequencedcontig.Weestimatethat thepanelcontains,6,000deletionbinsforD-genomechromosomesandwillrequire,18,000markersforhighresolution mapping.Map-baseddeletionestimatesrevealedamajorityof1–20Mbinterstitialdeletionssuggestingmutagenicrepair ofdouble-strandbreaksinpollenprovidesausefulresourceforRHmappingandmapbasedcloningstudies. Citation:TiwariVK,Riera-LizarazuO,GunnHL,LopezK,IqbalMJ,etal.(2012)EndospermToleranceofPaternalAneuploidyAllowsRadiationHybridMappingof theWheatD-GenomeandaMeasureofcRay-InducedChromosomeBreaks.PLoSONE7(11):