Estrogen Regulates Estrogen Receptors and Antioxidant Gene Expression in Mouse Skeletal Muscle 英文参考文献.docVIP

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Estrogen Regulates Estrogen Receptors and Antioxidant Gene Expression in Mouse Skeletal Muscle 英文参考文献.doc

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Estrogen Regulates Estrogen Receptors and Antioxidant Gene Expression in Mouse Skeletal Muscle 英文参考文献

EstrogenRegulatesEstrogenReceptorsandAntioxidant GeneExpressioninMouseSkeletalMuscle KristenA.Baltgalvis1*,SarahM.Greising2,GordonL.Warren3,DawnA.Lowe2 1Department of Biochemistry, Molecular Biology, and Biophysics, Medical School, University of Minnesota, Minneapolis, Minnesota, United States of America, 2DepartmentofPhysicalMedicineandRehabilitation,MedicalSchool,UniversityofMinnesota,Minneapolis,Minnesota,UnitedStatesofAmerica,3DivisionofPhysical Therapy,GeorgiaStateUniversity,Atlanta,Georgia,UnitedStatesofAmerica Abstract Background: Estrogens are associated with the loss of skeletal muscle strength in women with age. Ovarian hormone removalbyovariectomyinmiceleadstoalossofmusclestrength,whichisreversedwith17b-estradiolreplacement.Aging isalsoassociatedwithanincreaseinantioxidantstress,andestrogenscanimproveantioxidantstatusviatheirinteraction withestrogenreceptors(ER)toregulateantioxidantgeneexpression.ThepurposeofthisstudywastodetermineifERand antioxidant gene expression in skeletal muscle are responsive to changes in circulating estradiol, and if ERs regulate antioxidantgeneexpressioninthistissue. Methodology/PrincipalFindings:AdultC57BL/6miceunderwentovariectomiesorshamsurgeriestoremovecirculating estrogens.Thesemicewereimplantedwithplaceboor17b-estradiolpelletsacutelyorchronically.Aseparateexperiment examined mice that received weekly injections of Faslodex to chronically block ERs. Skeletal muscles were analyzed for expressionofERgenesandproteinsandantioxidantgenes.ERawasthemostabundant,followedbyGperandERbinboth soleusandEDLmuscles.ThelossofestrogensthroughovariectomyinducedERageneandproteinexpressioninthesoleus, EDL,andTAmusclesatboththeacuteandchronictimepoints.Gpx3mRNAwasalsoinducedbothacutelyandchronically inall3musclesinmicereceiving17b-estradiol.WhenERswereblockedusingFaslodex,Gpx3mRNAwasdownregulatedin thesoleusmuscle,butnottheEDLandTAmuscles. Conclusions/Significance:ThesedatasuggestthatGpx3andERageneexpressionaresensitivetocirculatingestrogensi