Evaluation of the Human IgG Antibody Response to Aedes albopictus Saliva as a New Specific Biomarker of Exposure to Vector Bites 英文参考文献.docVIP
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Evaluation of the Human IgG Antibody Response to Aedes albopictus Saliva as a New Specific Biomarker of Exposure to Vector Bites 英文参考文献
EvaluationoftheHumanIgGAntibodyResponseto
AedesalbopictusSalivaasaNewSpecificBiomarkerof
ExposuretoVectorBites
SouleymaneDoucoure1*,Franc?oisMouchet1,SylvieCornelie1,JeanSe′bastienDeHecq2,
AbdulHamidRutee2,YelinRoca3,AnnieWalter1,JeanPierreHerve′1,Dorothe′eMisse1,Franc?oisFavier4,
PhilippeGasque5,FranckRemoue1
1Laboratoire Maladies Infectieuses et Vecteurs: Ecologie, Ge′ne′tique, Evolution et Contro?le, UMR 224 CNRS/IRD/UM1, Montpellier, France, 2Direction Re′gionale des
AffairesSanitairesetSociale, SaintDenis,LaRe′union,France,3CentroNacionaldeEnfermedades,SantaCruz,Bolivia,4Centred’InvestigationClinique-Epide′miologie
Clinique,SaintPierre,LaRe′union, France,5GroupedeRechercheImmunopathologieetMaladieInfectieuses,SaintDenis,LaRe′union,France
Abstract
Background: The spread of Aedes albopictus, a vector for re-emergent arbovirus diseases like chikungunya and dengue,
pointsuptheneedforbettercontrolstrategiesandnewtoolstoevaluatetransmissionrisk.Humanantibody(Ab)responses
tomosquitosalivaryproteinscouldrepresentareliablebiomarkerforevaluatinghuman-vectorcontactandtheefficacyof
controlprograms.
Methodology/PrincipalFindings:WeusedELISAteststoevaluatespecificimmunoglobulinG(IgG)responsestosalivary
glandextracts(SGE)inadultsexposedtoAedesalbopictusinReunionIsland.Thepercentageofimmuneresponders(88%)
andlevelsofanti-SGEIgGAbswerehighinexposedindividuals.Atanindividuallevel,ourresultsindicateheterogeneityof
theexposuretoAedesalbopictusbites.Inaddition,low-levelimmunecross-reactivitybetweenAedesalbopictusandAedes
aegyptiSGEswasobserved,mainlyinthehighestresponders.
Conclusion/Significance:Abresponsestosalivacouldbeusedasanimmuno-epidemiologicaltoolforevaluatingexposure
toAedesalbopictusbites.Combinedwithentomologicalandepidemiologicalmethods,a‘‘salivary’’biomarkerofexposure
toAedesalbopictuscouldenhancesurveillanceofitsspreadandtheriskofarbovirustransmission,andcouldbeusedasa
directtoolfortheevaluationofAedesalbopictuscontrolstrategies.
Citation:DoucoureS,MouchetF,Corn
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