Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga 英文参考文献.docVIP

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Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga 英文参考文献.doc

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Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga 英文参考文献

ExaminationofTriacylglycerolBiosyntheticPathwaysvia DeNovoTranscriptomicandProteomicAnalysesinan UnsequencedMicroalga MichaelT.Guarnieri1*,AmbarishNag2,SharonL.Smolinski3,AlDarzins1¤,MichaelSeibert4,PhilipT. Pienkos1 1NationalBioenergyCenter,NationalRenewableEnergyLaboratory,Golden,Colorado,UnitedStatesofAmerica,2ComputationalScienceCenter,NationalRenewable EnergyLaboratory,Golden,Colorado,UnitedStatesofAmerica,3BiosciencesCenter,NationalRenewableEnergyLaboratory,Golden,Colorado,UnitedStatesofAmerica, 4EnergySciencesDirectorate,NationalRenewableEnergyLaboratory,Golden,Colorado,UnitedStatesofAmerica Abstract Biofuels derived from algal lipids represent an opportunity to dramatically impact the global energy demand for transportationfuels.Systemsbiologyanalysesofoleaginousalgaecouldgreatlyacceleratethecommercializationofalgal- derived biofuels by elucidating the key components involved in lipid productivity and leading to the initiation of hypothesis-drivenstrain-improvementstrategies.However,higher-levelsystemsbiologyanalyses,suchastranscriptomics andproteomics,arehighlydependentuponavailablegenomicsequencedata,andthelackofthesedatahashinderedthe pursuitofsuchanalysesformanyoleaginousmicroalgae.Inordertoexaminethetriacylglycerolbiosyntheticpathwayinthe unsequencedoleaginousmicroalga,Chlorellavulgaris,wehaveestablishedastrategywithwhichtobypassthenecessityfor genomicsequenceinformationbyusingthetranscriptomeasaguide.Ourresultsindicateanupregulationofbothfatty acidandtriacylglycerolbiosyntheticmachineryunderoil-accumulatingconditions,anddemonstratetheutilityofadenovo assembledtranscriptomeasasearchmodelforproteomicanalysisofanunsequencedmicroalga. Citation:GuarnieriMT,NagA,SmolinskiSL,DarzinsA,SeibertM,etal.(2011)ExaminationofTriacylglycerolBiosyntheticPathwaysviaDeNovoTranscriptomic andProteomicAnalysesinanUnsequencedMicroalga.PLoSONE6(10):e25851.doi:10.1371/journal.pone.0025851 Editor:DebashishBhattacharya,RutgersUniversity,UnitedStatesofAmerica ReceivedJuly1,2011;