Exploring the Diversity of Gardnerella vaginalis in the Genitourinary Tract Microbiota of Monogamous Couples Through Subtle Nucleotide Variation 英文参考文献.docVIP

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Exploring the Diversity of Gardnerella vaginalis in the Genitourinary Tract Microbiota of Monogamous Couples Through Subtle Nucleotide Variation 英文参考文献.doc

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Exploring the Diversity of Gardnerella vaginalis in the Genitourinary Tract Microbiota of Monogamous Couples Through Subtle Nucleotide Variation 英文参考文献

ExploringtheDiversityofGardnerellavaginalisinthe GenitourinaryTractMicrobiotaofMonogamousCouples ThroughSubtleNucleotideVariation A.MuratEren1,2*,MarcelaZozaya3,ChristopherM.Taylor1,3,ScotE.Dowd4,DavidH.Martin5,MichaelJ. Ferris3,6 1DepartmentofComputerScience,UniversityofNewOrleans,NewOrleans,Louisiana,UnitedStatesofAmerica,2JosephineBayPaulCenterforComparativeMolecular BiologyandEvolution,MarineBiologicalLaboratory,WoodsHole,Massachusetts,UnitedStatesofAmerica,3TheResearchInstituteforChildren,Children’sHospital,New Orleans,Louisiana,UnitedStatesofAmerica, 4ResearchandTestingLaboratory,Lubbock, Texas,UnitedStatesofAmerica,5SectionofInfectiousDiseases,Louisiana StateUniversityHealthSciencesCenter,NewOrleans,Louisiana,UnitedStatesofAmerica,6DepartmentsofPediatricsandMicrobiologyImmunologyandParasitology, LouisianaStateUniversityHealthSciencesCenter,NewOrleans,Louisiana,UnitedStatesofAmerica Abstract Background:Bacterialvaginosis(BV)isanenigmaticdiseaseofunknownoriginthataffectsalargepercentageofwomen. ThevaginalmicrobiotaofwomenwithBVisassociatedwithserioussequelae,includingabnormalpregnancies.Theetiology of BV is not fully understood, however, it has been suggested that it is transmissible, and that G. vaginalis may be an etiological agent. Studies using enzymatic assays to define G. vaginalis biotypes, as well as more recent genomic comparisonsofG.vaginalisisolatesfromsymptomaticandasymptomaticwomen,suggestthatparticularG.vaginalisstrains mayplayakeyroleinthepathogenesisofBV. Methodology/Principal Findings: To explore G. vaginalis diversity, distribution andsexual transmission, we developeda Shannonentropy-based methodtoanalyzelow-level sequencevariationin 65,710 G. vaginalis16SrRNA genesegments thatwerePCR-amplifiedfromvaginalsamplesof53monogamouswomenandfromurethralandpenileskinsamplesof their male partners. We observed a high degree of low-level diversity among G. vaginalis sequences with a total of 46 unique sequence variants (oligotypes), and also foun