Expression of Human Beta-Defensins in Children with Chronic Inflammatory Bowel Disease 英文参考文献.docVIP

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Expression of Human Beta-Defensins in Children with Chronic Inflammatory Bowel Disease 英文参考文献.doc

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Expression of Human Beta-Defensins in Children with Chronic Inflammatory Bowel Disease 英文参考文献

ExpressionofHumanBeta-DefensinsinChildrenwith ChronicInflammatoryBowelDisease MatthiasZilbauer1,2*,AndreasJenke1,GundulaWenzel1,JanPostberg1,AndreasHeusch1,AlanD. Phillips3,GabrieleNoble-Jamieson2,FrancoTorrente2,CamillaSalvestrini2,RobertHeuschkel2 ,Stefan Wirth1 1DepartmentofPaediatrics,HELIOSKlinikumWuppertal,Germany,2DepartmentofPaediatricGastroenterology,Addenbrooke’sHospital,Cambridge,UnitedKingdom, 3CentreforPaediatricGastroenterology,RoyalFreeHospital,London,UnitedKingdom Abstract Background:Humanbeta-defensins(hBDs)areantimicrobialpeptidesknowntoplayamajorroleinintestinalinnatehost defence.AlteredmucosalexpressionofhBDshasbeensuggestedtobeimplicatedinchronicinflammatoryboweldisease pathogenesis.However,littleisknownaboutexpressionofthesepeptidesinchildren. Methods: Intestinal biopsies were obtained from the duodenum (n=88), terminal ileum (n=90) and ascending colon (n=105)ofchildrenwithCrohn’sdisease(n=26),ulcerativecolitis(n=11)andhealthycontrols(n=16).Quantitativereal- time(RT)PCRwasperformedandabsolutemRNAcopynumbersanalyzedforhBD1-3aswellasinflammatorycytokinesIL-8 andTNF-alpha. Results:SignificantinductionofhBD2andhBD3wasobservedintheinflamedterminalileumandascendingcolonofIBD children. In the ascending colon induction of hBD2 was found to be significantly lower in children with Crohn’s disease compared to ulcerative colitis. A strong correlation was found between inducible defensins hBD2 and 3 and the inflammatorycytokinesIL-8andTNF-alpha,bothintheterminalileumandascendingcolon. Conclusion:OurstudydemonstratesdistinctchangesinhBDexpressionthroughouttheintestinaltractofchildrenwithIBD, lendingfurthersupportfortheirpotentialroleindiseasepathogenesis. Citation:ZilbauerM,JenkeA,WenzelG,PostbergJ,HeuschA,etal.(2010)ExpressionofHumanBeta-DefensinsinChildrenwithChronicInflammatoryBowel Disease.PLoSONE5(10):e15389.doi:10.1371/journal.pone.0015389 Editor:StefanBereswill,Charite′-Universita¨tsmedizin Berlin,Germany ReceivedAugust20,2010;AcceptedSeptember6,2010