Expression of Measles Virus Nucleoprotein Induces Apoptosis and Modulates Diverse Functional Proteins in Cultured Mammalian Cells 英文参考文献.docVIP

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Expression of Measles Virus Nucleoprotein Induces Apoptosis and Modulates Diverse Functional Proteins in Cultured Mammalian Cells 英文参考文献.doc

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Expression of Measles Virus Nucleoprotein Induces Apoptosis and Modulates Diverse Functional Proteins in Cultured Mammalian Cells 英文参考文献

ExpressionofMeaslesVirusNucleoproteinInduces ApoptosisandModulatesDiverseFunctionalProteinsin CulturedMammalianCells AshimaBhaskar,JyotiBala,AkhilVarshney,PramodYadava* AppliedMolecularBiologyLaboratory,SchoolofLifeSciences,JawaharlalNehruUniversity,NewDelhi,India Abstract Background:Measlesvirusnucleoprotein(N)encapsidatestheviralRNA,protectsitfromendonucleasesandformsavirus specific template for transcription and replication. It is the most abundant protein during viral infection. Its C-terminal domainisintrinsicallydisorderedimpartingittheflexibilitytointeractwithseveralcellularandviralpartners. PrincipalFindings:Inthisstudy,wedemonstratethatexpressionofNwithinmammaliancellsresultedinmorphological transitions, nuclear condensation, DNAfragmentation andactivation of Caspase 3 eventuatinginto apoptosis. Therapid generationofintracellularreactiveoxygenspecies(ROS)wasinvolvedinthemechanismofcelldeath.Additionofascorbic acid(AA)orinhibitorofcaspase-3intheextracellularmediumpartiallyreversedNinducedapoptosis.Wealsostudiedthe proteinprofileofcellsexpressingNprotein.MSanalysisrevealedthedifferentialexpressionof25proteinsoutofwhich11 proteinswereupregulatedwhile14showsignsofdownregulationuponNexpression.2DEresultswerevalidatedbyreal timeandsemiquantitativeRT-PCRanalysis. Conclusion:Theseresultsshowthepro-apoptoticeffectsofNindicatingitspossibledevelopmentasanapoptogenictool. Our2DEresultspresentprimafacieevidencethattheMVnucleoproteininteractswithorcausesdifferentialexpressionofa wide rangeof cellular factors. Atthis stageitis notclear as towhat the adaptive response of thehost cell is andwhat reflectsastrategicmodulationexertedbythevirus. Citation:BhaskarA,BalaJ,VarshneyA,YadavaP(2011)ExpressionofMeaslesVirusNucleoproteinInducesApoptosisandModulatesDiverseFunctionalProteins inCulturedMammalianCells.PLoSONE6(4):e18765.doi:10.1371/journal.pone.0018765 Editor:RaymondJ.Pickles,UniversityofNorthCarolinaatChapelHill,UnitedStatesofAmerica ReceivedOctober1,2010;AcceptedMarch16