Expression of PPARγ and Paraoxonase 2 Correlated with Pseudomonas aeruginosa Infection in Cystic Fibrosis 英文参考文献.docVIP

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Expression of PPARγ and Paraoxonase 2 Correlated with Pseudomonas aeruginosa Infection in Cystic Fibrosis 英文参考文献.doc

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Expression of PPARγ and Paraoxonase 2 Correlated with Pseudomonas aeruginosa Infection in Cystic Fibrosis 英文参考文献

ExpressionofPPARcandParaoxonase2Correlatedwith PseudomonasaeruginosaInfectioninCysticFibrosis PhoebeE.Griffin1,2,LouiseF.Roddam2,YvonneC.Belessis3,RoxanneStrachan3,SeanBeggs2,4, AdamJaffe3,MargaretA.Cooley2* 1Menzies Research Institute, Hobart, Tasmania, Australia, 2School of Medicine, University of Tasmania, Hobart, Tasmania, Australia, 3Department of Respiratory Medicine,SydneyChildren’sHospital,Randwick,NewSouthWales,Australia,4DepartmentofPediatrics,RoyalHobartHospital,Hobart,Tasmania,Australia Abstract The Pseudomonas aeruginosa quorum sensing signal molecule N-3-oxododecanoyl-L-homoserine lactone (3OC12HSL) can inhibit function of the mammalian anti-inflammatory transcription factor peroxisome proliferator activated receptor (PPAR)c,andcanbedegradedbyhumanparaoxonase(PON)2.Because3OC12HSLisdetectedinlungsofcysticfibrosis(CF) patientsinfectedwithP.aeruginosa,weinvestigatedtherelationshipbetweenP.aeruginosainfectionandgeneexpression ofPPARcandPON2inbronchoalveolarlavagefluid(BALF)ofchildrenwithCF.TotalRNAwasextractedfromcellpelletsof BALFfrom43childrenaged6months–5yearsandanalyzedbyreversetranscription–quantitativerealtimePCRforgene expressionofPPARc,PON2,andP.aeruginosalasI,the3OC12HSLsynthase.Patientswithculture-confirmedP.aeruginosa infection had significantly lower gene expression of PPARc and PON2 than patients without P. aeruginosa infection. All samples that were culture-positive for P. aeruginosa were also positive for lasI expression. There was no significant difference in PPARc or PON2 expression between patients without culture-detectable infection and those with non- Pseudomonalbacterialinfection,soreducedexpressionwasspecificallyassociatedwithP.aeruginosainfection.Expression ofbothPPARcandPON2wasinverselycorrelatedwithneutrophilcountsinBALF,butshowednocorrelationwithother variablesevaluated.Thus,lowerPPARcandPON2geneexpressionintheBALFofchildrenwithCFisassociatedspecifically withP.aeruginosainfectionandneutrophilia.Wecannotdifferentiatewhetherthis