Expression of Paracoccidioides brasiliensis AMY1 in a Histoplasma capsulatum amy1 Mutant, Relates an α-(1,4)-Amylase to Cell Wall α-(1,3)-Glucan Synthesis 英文参考文献.docVIP

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Expression of Paracoccidioides brasiliensis AMY1 in a Histoplasma capsulatum amy1 Mutant, Relates an α-(1,4)-Amylase to Cell Wall α-(1,3)-Glucan Synthesis 英文参考文献.doc

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Expression of Paracoccidioides brasiliensis AMY1 in a Histoplasma capsulatum amy1 Mutant, Relates an α-(1,4)-Amylase to Cell Wall α-(1,3)-Glucan Synthesis 英文参考文献

ExpressionofParacoccidioidesbrasiliensisAMY1in aHistoplasmacapsulatumamy1Mutant,Relatesana- (1,4)-AmylasetoCellWalla-(1,3)-GlucanSynthesis EmmaCamacho1,VictoriaE.Sepulveda2,3,WilliamE.Goldman2,GiocondaSan-Blas1,GustavoA.Nin?o- Vega1* 1CentrodeMicrobiolog?′ayBiolog?′aCelular,InstitutoVenezolanodeInvestigacionesCient?′ficas,Caracas,Venezuela, 2DepartmentofMicrobiologyandImmunology, UniversityofNorthCarolinaatChapelHill,ChapelHill,NorthCarolina,UnitedStatesofAmerica,3DepartmentofMolecularMicrobiology,WashingtonUniversityinSt. Louis,St.Louis,Missouri,UnitedStatesofAmerica Abstract In thecell walls of thepathogenic yeast phases of Paracoccidioides brasiliensis, Blastomyces dermatitidis andHistoplasma capsulatum,theoutera-(1,3)-glucanlayerbehavesasavirulencefactor.InH.capsulatum,ana-(1,4)-amylasegene(AMY1 )is essentialforthesynthesisofthispolysaccharide,hencerelatedtovirulence.AnorthologousgenetoH.capsulatumAMY1 wasidentifiedinP.brasiliensisandalsolabeledAMY1.P.brasiliensisAMY1transcriptionallevelswereincreasedduringthe yeast phase, which correlates with the presence of a-(1,3)-glucan as the major yeast cell wall polysaccharide. Complementation of a H. capsulatum amy1 mutant strain with P. brasiliensis AMY1, suggests that P. brasiliensis Amy1p mayplayaroleinthesynthesisofcellwalla-(1,3)-glucan.TostudysomebiochemicalpropertiesofP.brasiliensisAmy1p,the enzyme was overexpressed, purified and studied its activity profile with starch and amylopeptin. It showed a relatively higher hydrolyzing activity on amylopeptin than starch, producing oligosaccharides from 4 to 5 glucose residues. Our findingsshowthatP.brasiliensisAmy1pproducesmaltooligosaccharideswhichmayactasaprimermoleculeforthefungal cellwalla-(1,3)-glucanbiosynthesisbyAgs1p. Citation:CamachoE,SepulvedaVE,GoldmanWE,San-BlasG,Nin?o-VegaGA(2012)ExpressionofParacoccidioidesbrasiliensisAMY1inaHistoplasmacapsulatum amy1Mutant,Relatesana-(1,4)-AmylasetoCellWalla-(1,3)-GlucanSynthesis.PLoSONE7(11):e50201.doi:10.1371/