Expression Profile of MicroRNAs in Young Stroke Patients 英文参考文献.docVIP

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Expression Profile of MicroRNAs in Young Stroke Patients 英文参考文献.doc

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Expression Profile of MicroRNAs in Young Stroke Patients 英文参考文献

ExpressionProfileofMicroRNAsinYoungStrokePatients KaySinTan1,ArunmozhiarasiArmugam2,SugunavathiSepramaniam2,KaiYingLim2,KarolinaDwi Setyowati2,CheeWoonWang1,KandiahJeyaseelan2* 1Departments of Medicine and Molecular Medicine, University of Malaya, Kuala Lumpur, Malaysia, 2Department of Biochemistry, Yong Loo Lin School of Medicine, NationalUniversityofSingapore,Singapore,Singapore Abstract Background: The methods currently available for diagnosis and prognosis of cerebral ischaemia still require further improvements.Micro-RNAs(smallnon-codingRNAs)havebeenrecentlyreportedasusefulbiomarkersindiseasessuchas cancer and diabetes. We therefore carried out microRNA (miRNA) profiling from peripheral blood to detect and identify characteristicpatternsinischaemicstroke. Methods/Principal Findings: The ischaemic stroke patients aged between 18–49 years, characterized based on World HealthOrganizationclinicalcriteriawerefurtherclassifiedaccordingtoTOASTclassification,a)Large-vesselatherosclerosis [n=8]b)Small-vesseldisease[n=3]c)Cardioembolism[n=5]d)Undeterminedcause[n=3].Thepatients’functionalstatus atthetimeofbloodsampling(attheoutpatientclinics)wasevaluatedwiththemodifiedRankinScale(mRS).Bloodsamples fromnormal(n=5)individualswereusedascontrols.TotalRNAextractedfromwholebloodwassubjectedtomiroRNA profiling and real-time PCR analysis. miRNAs that are implicated in the endothelial/vascular function, erythropoiesis, angiogenesisandneuralfunctionshoweddifferentialexpressionprofileascomparedtothenormalcontrol.Interestingly, miRNAsthatareinvolvedinhypoxicconditionshavealsobeenfoundinourmiRNAprofiles. Conclusion: We demonstrate that the peripheral blood miRNAs and their profiles can be developed as biomarkers in diagnosisandprognosisofcerebralischaemicstroke.ThedysregulatedmiRNAshavebeendetectableevenafterseveral monthsfromtheonsetofstrokeinwhatisusuallyregardedasneurologicallystablepatients. Citation:TanKS,ArmugamA,SepramaniamS,LimKY,SetyowatiKD,etal.(2009)ExpressionProfileofMic