Extensive Conserved Synteny of Genes between the Karyotypes of Manduca sexta and Bombyx mori Revealed by BAC-FISH Mapping 英文参考文献.docVIP

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Extensive Conserved Synteny of Genes between the Karyotypes of Manduca sexta and Bombyx mori Revealed by BAC-FISH Mapping 英文参考文献.doc

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Extensive Conserved Synteny of Genes between the Karyotypes of Manduca sexta and Bombyx mori Revealed by BAC-FISH Mapping 英文参考文献

ExtensiveConservedSyntenyofGenesbetweenthe KaryotypesofManducasextaandBombyxmori RevealedbyBAC-FISHMapping YujiYasukochi1.*,MakikoTanaka-Okuyama1,FukashiShibata2,AtsuoYoshido2,Frantisˇek Marec3, ChengcangWu4¤,HongbinZhang4,MarianR.Goldsmith5,KenSahara2. 1InsectGenomeResearchUnit,NationalInstituteofAgrobiologicalSciences,Tsukuba,Ibaraki,Japan,2LaboratoryofAppliedMolecularEntomology,GraduateSchoolof Agriculture, Hokkaido University, Sapporo, Japan, 3Institute of Entomology, Biology Centre ASCR, Ceskeˇ ′ Budejovice, Czech Republic, 4Department of Soil and Crop Sciences,TexasAMUniversity,CollegeStation,Texas,UnitedStatesofAmerica,5BiologicalSciencesDepartment,UniversityofRhodeIsland,Kingston,RhodeIsland, UnitedStatesofAmerica ˇ Abstract Background: Genome sequencing projects have been completed for several species representing four highly diverged holometabolous insect orders, Diptera, Hymenoptera, Coleoptera,and Lepidoptera.The striking evolutionary diversity of insectsarguesaneedforefficientmethodstoapplygenomeinformationfromsuchmodelstogeneticallyuncharacterized species.Constructingconservedsyntenymapsplaysacrucialroleinthistask.Here,wedemonstratetheuseoffluorescence in situ hybridization with bacterial artificial chromosome probes as a powerful tool for physical mapping of genes and comparative genome analysis in Lepidoptera, which have numerous and morphologically uniform holokinetic chromosomes. Methodology/PrincipalFindings:Weisolated214clonescontaining159orthologsofwellconservedsingle-copygenesof asequencedlepidopteranmodel,thesilkworm,Bombyxmori,fromaBAClibraryofasphingidwithanunexploredgenome, thetobaccohornworm,Manducasexta.WethenconstructedaBAC-FISHkaryotypeidentifyingall28chromosomesofM. sexta by mapping 124 loci using the corresponding BAC clones. BAC probes from three M. sexta chromosomes also generated clear signals on the corresponding chromosomes of the convolvulus hawk moth, Agrius convolvuli , which belongstothesamesubfamily,Sphinginae,asM