Exploring New Biological Functions of Amyloids Bacteria Cell Agglutination Mediated by Host Protein Aggregation 英文参考文献.docVIP
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Exploring New Biological Functions of Amyloids Bacteria Cell Agglutination Mediated by Host Protein Aggregation 英文参考文献
ExploringNewBiologicalFunctionsofAmyloids:Bacteria
CellAgglutinationMediatedbyHostProtein
Aggregation
MarcTorrent1,2*,DavidPulido1,M.Victo`riaNogue′s1,EsterBoix1*
1Department of Biochemistry and Molecular Biology, Biosciences Faculty, Universitat Auto`noma de Barcelona, Cerdanyola del Valle`s, Spain, 2Department of
ExperimentalandHealthSciences,UniversitatPompeuFabra,BarcelonaBiomedicalResearchPark,Barcelona,Spain
Abstract
Antimicrobialproteinsandpeptides(AMPs)areimportanteffectorsoftheinnateimmunesystemthatplayavitalroleinthe
preventionofinfections.RecentadvanceshavehighlightedthesimilaritybetweenAMPsandamyloidproteins.Usingthe
Eosinophil Cationic Protein as a model, we have rationalized the structure-activity relationships between amyloid
aggregationandantimicrobialactivity.Ourresultsshowhowproteinaggregationcaninducebacteriaagglutinationand
celldeath. Usingconfocal andtotalinternal reflection fluorescence microscopywehavetracked theformation insitu of
proteinamyloid-likeaggregatesatthebacteriasurfaceandonmembranemodels.Inbothcases,fibrillaraggregatesableto
bindtoamyloiddiagnosticdyesweredetected.Additionally,asinglepointmutation(Ile13toAla)cansuppresstheprotein
amyloidbehavior,abolishingtheagglutinatingactivityandimpairingtheantimicrobialaction.Themutantisalsodefective
in triggering both leakage and lipid vesicle aggregation. We conclude that ECP aggregation at the bacterial surface is
essentialforitscytotoxicity.Hence,weproposehereanewprospectivebiologicalfunctionforamyloid-likeaggregateswith
potentialbiologicalrelevance.
Citation:TorrentM,PulidoD,Nogue′s MV,BoixE(2012)ExploringNewBiologicalFunctionsofAmyloids:BacteriaCellAgglutinationMediatedbyHostProtein
Aggregation.PLoSPathog8(11):e1003005.doi:10.1371/journal.ppat.1003005
Editor:H.StevenSeifert,NorthwesternUniversityFeinbergSchoolofMedicine,UnitedStatesofAmerica
ReceivedMay28,2012;AcceptedSeptember17,2012;PublishedNovember1,2012
Copyright: ? 2012 Torrent et al. This is an open-access article distribute
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