Fast Growth Increases the Selective Advantage of a Mutation Arising Recurrently during Evolution under Metal Limitation 英文参考文献.docVIP

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Fast Growth Increases the Selective Advantage of a Mutation Arising Recurrently during Evolution under Metal Limitation 英文参考文献.doc

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Fast Growth Increases the Selective Advantage of a Mutation Arising Recurrently during Evolution under Metal Limitation 英文参考文献

FastGrowthIncreasestheSelectiveAdvantageofa MutationArisingRecurrentlyduringEvolutionunder MetalLimitation Hsin-HungChou,JuliaBerthet¤,ChristopherJ.Marx* DepartmentofOrganismicandEvolutionaryBiology,HarvardUniversity,Cambridge,Massachusetts,UnitedStatesofAmerica Abstract Understandingtheevolutionofbiologicalsystemsrequiresuntanglingthemolecularmechanismsthatconnectgeneticand environmental variations to their physiological consequences. Metal limitation across many environments, ranging from pathogensinthehumanbodytophytoplanktonintheoceans,imposesstrongselectionforimprovedmetalacquisition systems. In this study, we uncovered the genetic and physiological basis of adaptation to metal limitation using experimentalpopulationsofMethylobacterium extorquens AM1evolvedinmetal-deficientgrowthmedia.Weidentifieda transpositionmutationarisingrecurrentlyin30of32independentpopulationsthatutilizedmethanolasacarbonsource, but not in any of the 8 that utilized only succinate. These parallel insertion events increased expression of a novel transportersystemthatenhancedcobaltuptake.SuchabilityensuredtheproductionofvitaminB12,acobalt-containing cofactor,tosustaintwovitaminB12–dependentenzymaticreactionsessentialtomethanol,butnotsuccinate,metabolism. Interestingly,thismutationprovidedhigherselectiveadvantagesundergeneticbackgroundsorincubationtemperatures that permit faster growth, indicating growth-rate–dependent epistatic and genotype-by-environment interactions. Our results link beneficial mutations emerging in a metal-limiting environment to their physiological basis in carbon metabolism,suggestthatcertainmolecularfeaturesmaypromotetheemergenceofparallelmutations,andindicatethat theselectiveadvantagesofsomemutationsdependgenericallyuponchangesingrowthratethatcanstemfromeither geneticorenvironmentalinfluences. Citation:ChouH-H,BerthetJ,MarxCJ(2009)FastGrowthIncreasestheSelectiveAdvantageofaMutationArisingRecurrentlyduringEvolutionunderMetal Limitation.PLoSGenet5(9):e1000652.doi:1