网站大量收购闲置独家精品文档,联系QQ:2885784924

Fatal Outcome in Bacteremia is Characterized by High Plasma Cell Free DNA Concentration and Apoptotic DNA Fragmentation A Prospective Cohort Study 英文参考文献.docVIP

Fatal Outcome in Bacteremia is Characterized by High Plasma Cell Free DNA Concentration and Apoptotic DNA Fragmentation A Prospective Cohort Study 英文参考文献.doc

  1. 1、本文档共8页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
  5. 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
  6. 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们
  7. 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
  8. 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Fatal Outcome in Bacteremia is Characterized by High Plasma Cell Free DNA Concentration and Apoptotic DNA Fragmentation A Prospective Cohort Study 英文参考文献

FatalOutcomeinBacteremiaisCharacterizedbyHigh PlasmaCellFreeDNAConcentrationandApoptoticDNA Fragmentation:AProspectiveCohortStudy ReettaHuttunen1,2*,TaruKuparinen3,JuuliaJylha¨va¨3,JanneAittoniemi4,RistoVuento4,HeiniHuhtala5, JanneLaine1,JaanaSyrja¨nen1,2,MikkoHurme3,4 1Department of Internal Medicine, Tampere University Hospital, Tampere, Finland, 2University of Tampere Medical School, Tampere, Finland, 3Department of MicrobiologyandImmunology,UniversityofTampereMedicalSchool,Tampere,Finland,4CentreforLaboratoryMedicine,PirkanmaaHospitalDistrict,Tampere,Finland, 5SchoolofHealthSciences,UniversityofTampere,Tampere,Finland Abstract Introduction:Recentstudieshaveshownthatapoptosisplaysacriticalroleinthepathogenesisofsepsis.Highplasmacell freeDNA(cf-DNA)concentrationshavebeenshowntobeassociatedwithsepsisoutcome.Theoriginofcf-DNAisunclear. Methods:Totalplasmacf-DNAwasquantifieddirectlyinplasmaandtheamplifiablecf-DNAassessed usingquantitative PCR in 132 patients with bacteremia caused by Staphylococcus aureus, Streptococcus pneumoniae , ?-hemolytic streptococcae or Escherichia coli. The quality of cf-DNA was analyzed with a DNA Chip assay performed on 8 survivors and8nonsurvivors.Valuesweremeasuredondays1–4afterpositivebloodculture,onday5–17andonrecovery. Results:Themaximumcf-DNAvaluesondays1–4(n=132)weremarkedlyhigherinnonsurvivorscomparedtosurvivors (2.03vs1.26ug/ml,p,0.001)andtheAUCROCinthepredictionofcasefatalitywas0.81(95%CI0.69–0.94).cf-DNAatacut- offlevelof1.52ug/mlshowed83%sensitivityand79%specificityforfataldisease.Highcf-DNA(.1.52ug/ml)remainedan independentriskfactorforcasefatalityinalogisticregressionmodel.Qualitativeanalysisofcf-DNAshowedthatcf-DNA displayedapredominatinglow-molecular-weightcf-DNAband(150–200bp)innonsurvivors,correspondingtothesizeof the apoptotic nucleosomal DNA. cf-DNA concentration showed a significant positive correlation with visually graded apoptoticbandintensity(R=0.822,p,0.001). Conclusions: Plasma cf-DNA concentration pro

您可能关注的文档

文档评论(0)

sheppha + 关注
实名认证
文档贡献者

该用户很懒,什么也没介绍

版权声明书
用户编号:5134022301000003

1亿VIP精品文档

相关文档