Feeding-Based RNA Intereference of a Gap Gene Is Lethal to the Pea Aphid, Acyrthosiphon pisum 英文参考文献.docVIP

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Feeding-Based RNA Intereference of a Gap Gene Is Lethal to the Pea Aphid, Acyrthosiphon pisum 英文参考文献.doc

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Feeding-Based RNA Intereference of a Gap Gene Is Lethal to the Pea Aphid, Acyrthosiphon pisum 英文参考文献

Feeding-BasedRNAIntereferenceofaGapGeneIsLethal tothePeaAphid,Acyrthosiphonpisum JianjunMao,FanrongZeng* The Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s RepublicofChina Abstract Thegapgenehunchback(hb)isakeyregulatorintheanteroposteriorpatterningofinsects.Loss-of-functionofhbresulted in segmentation defects in the next generation. In this paper, hb expression level was investigated at different developmentalstagesofthepeaaphid,Acyrthosiphonpisum(Ap).AphbmRNAwasmostearlydetectedatthefirstinstar stageandshowedanincontinuousincreaseinthewholelifecycle.IngestedRNAinterferencewasperformedatthesecond instarstagetoknockdowntheAphbexpression.ContinuousfeedingofAphbdouble-strandedRNAmixedinartificialdiet ledtoreductionofAphbtranscripts andriseofinsectlethality.Theseresultsindicatedthat hunchbackwasagoodRNAi targetinthemanagementofinsectpests. Citation: Mao J, Zeng F (2012) Feeding-Based RNA Intereference of a Gap Gene Is Lethal to the Pea Aphid, Acyrthosiphon pisum. PLoS ONE 7(11): e48718. doi:10.1371/journal.pone.0048718 Editor:AndreVanWijnen,UniversityofMassachusettsMedical,UnitedStatesofAmerica ReceivedJuly17,2012;AcceptedSeptember28,2012;PublishedNovember7,2012 Copyright: ? 2012 Mao, Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbyProjectNSFndagriculturecooperativeresearchfund2011-G4.Thecontentissolelytheresponsibilityofthe authors.Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:Zengfr@ Introduction (Lepidoptera:Tortricidae),RNAiwastriggeredbyoraldeliveryof dsRNAtolarvaeandadult[8].IngestionofdsRN