Functional and Transcriptional Characterization of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction 英文参考文献.docVIP

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Functional and Transcriptional Characterization of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction 英文参考文献.doc

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Functional and Transcriptional Characterization of Human Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Myocardial Infarction 英文参考文献

FunctionalandTranscriptionalCharacterizationof HumanEmbryonicStemCell-DerivedEndothelialCells forTreatmentofMyocardialInfarction ZongjinLi1,7,KitchenerD.Wilson1,2,BryanSmith1,DanielL.Kraft3,FangjunJia1,MeiHuang1 ,Xiaoyan Xie1,RobertC.Robbins4,SanjivS.Gambhir1,IrvingL.Weissman3,5,JosephC.Wu1,5,6 * 1DepartmentofRadiologyandMolecularImagingProgramatStanford(MIPS),StanfordUniversitySchoolofMedicine,Stanford,California,UnitedStatesofAmerica, 2DepartmentofBioengineering,StanfordUniversitySchoolofMedicine,Stanford,California,UnitedStatesofAmerica,3DepartmentofDevelopmentalBiology,Stanford UniversitySchoolofMedicine,Stanford,California,UnitedStatesofAmerica,4DepartmentofCardiothoracicSurgery,StanfordUniversitySchoolofMedicine,Stanford, California,UnitedStatesofAmerica,5InstituteforStemCellBiologyandRegenerativeMedicine,StanfordUniversitySchoolofMedicine,Stanford,California,UnitedStates ofAmerica,6DepartmentofMedicine,DivisionofCardiology,StanfordUniversitySchoolofMedicine,Stanford,California,UnitedStatesofAmerica,7NankaiUniversity SchoolofMedicine,Tianjin,China Abstract Background:Differentiationofhumanembryonicstemcellsintoendothelialcells(hESC-ECs)hasthepotentialtoprovidean unlimited source of cells for novel transplantation therapies of ischemic diseases by supporting angiogenesis and vasculogenesis.However,theendothelialdifferentiationefficiencyoftheconventionalembryoidbody(EB)methodislow whilethe2-dimensionalmethodofco-culturingwithmouseembryonicfibroblasts(MEFs)requireanimalproduct,bothof whichcanlimitthefutureclinicalapplicationofhESC-ECs.Moreover,tofullyunderstandthebeneficialeffectsofstemcell therapy,investigatorsmustbeabletotrackthefunctionalbiologyandphysiologyoftransplantedcellsinlivingsubjects overtime. Methodology: In this study, we developed an extracellular matrix (ECM) culture system for increasing endothelial differentiation and free from contaminating animal cells. We investigated the transcriptional changes that occur during endothelialdifferentiationo