Functional Assessment of EnvZOmpR Two-Component System in Shewanella oneidensis 英文参考文献.docVIP

下载本文档

1
0
约4.72万字
约 9页
2017-05-11 发布于上海
举报
版权申诉

Functional Assessment of EnvZOmpR Two-Component System in Shewanella oneidensis 英文参考文献.doc

1、本文档共9页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Functional Assessment of EnvZOmpR Two-Component System in Shewanella oneidensis 英文参考文献

FunctionalAssessmentofEnvZ/OmpRTwo-Component SysteminShewanellaoneidensis JieYuan,BuyunWei,MiaomiaoShi,HaichunGao* InstituteofMicrobiology,CollegeofLifeSciences,ZhejiangUniversity,Hangzhou,Zhejiang,China Abstract EnvZ and OmpR constitute the bacterial two-component signal transduction system known to mediate osmotic stress response in a number of Gram-negative bacteria. In an effort to understand the mechanism through which Shewanella oneidensissensesandrespondstoenvironmentalosmolaritychanges,structureoftheompR-envZoperonwasdetermined with Northern blotting assay and roles of the EnvZ/OmpR two-component system in response to various stresses were investigatedwithmutationalanalysis,quantitativereversetranscriptasePCR(qRT-PCR),andphenotypemicroarrays.Results fromthemutationalanalysisandqRT-PCRsuggestedthattheEnvZ/OmpRsystemcontributedtoosmoticstressresponseof S. oneidensis and very likely engaged asimilar strategy employed by E. coli,which involved reciprocal regulation of two majorporincodinggenes.Additionally,theompR-envZsystemwasalsofoundrelatedtocellmotility.Wefurthershowed thattheompR-envZdependentregulationofporingenesandmotilityresidedalmostcompletelyonompRandonlypartially onenvZ,indicatingadditionalmechanismsforOmpRphosphorylation.IncontrasttoE.colilackingompR-envZ ,however, growthofS.oneidensisdidnotshowasignificantdependenceonompR-envZevenunderosmoticstress.Furtheranalysis with phenotype microarrays revealed that the S. oneidensis strains lacking a complete ompR-envZ system displayed hypersensitivitiestoanumberofagents,especiallyinalkalineenvironment.Takentogether,ourresultssuggestthatthe functionoftheompR-envZsysteminS.oneidensis,althoughstillconnectedwithosmoregulation,hasdivergedconsiderably fromthatofE.coli.AdditionalmechanismmustexisttosupportgrowthofS.oneidensisunderosmoticstress. Citation:YuanJ,WeiB,ShiM,GaoH(2011)FunctionalAssessmentofEnvZ/OmpRTwo-ComponentSysteminShewanellaoneidensis.PLoSONE6(8):e23701. doi:10.1371/journal.pone.0023701 Editor:Ma