Genetic and Physical Interactions between Tel2 and the Med15 Mediator Subunit in Saccharomyces cerevisiae 英文参考文献.docVIP

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Genetic and Physical Interactions between Tel2 and the Med15 Mediator Subunit in Saccharomyces cerevisiae 英文参考文献.doc

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Genetic and Physical Interactions between Tel2 and the Med15 Mediator Subunit in Saccharomyces cerevisiae 英文参考文献

GeneticandPhysicalInteractionsbetweenTel2andthe Med15MediatorSubunitinSaccharomycescerevisiae NathalieGrandin1,2,LaetitiaCorset2,MichelCharbonneau1,2* 1UMRCNRS5239,EcoleNormaleSupe′rieuredeLyon,IFR128BioSciencesGerland,Lyon,France,2UMRCNRS6239,Universite′ deTours,Tours,France Abstract Background:Inbuddingyeast,thehighlyconservedTel2proteinispartofseveralcomplexesanditsmainfunctionisnow believedtobeinthebiogenesisofphosphatidylinositol3-kinaserelatedkinases. PrincipalFindings:TouncoverpotentiallynovelfunctionsofTel2,wesetouttoisolatetemperature-sensitive(ts)mutant alleles of TEL2 in order to perform genetic screenings. MED15/GAL11, a subunit of Mediator, a general regulator of transcription, was isolated as a suppressor of these mutants. The isolated tel2 mutants exhibited a short telomere phenotype that was partially rescued by MED15/GAL11 overexpression. The tel2-15mutant was markedly deficient in the transcriptionofEST2,codingforthecatalyticsubunitoftelomerase,potentiallyexplainingtheshorttelomerephenotypeof thismutant.Inparallel,atwo-hybridscreenidentifiedanassociationbetweenTel2andRvb2,ahighlyconservedmemberof the AAA+ family of ATPases further found by in vivo co-immunoprecipitation to be tight and constitutive. Transiently overproduced Tel2 and Med15/Gal11 associated together, suggesting a potential role for Tel2 in transcription. Other Mediatorsubunits,aswellasSUA7/TFIIB,alsorescuedthetel2-tsmutants. Significance:Altogether,thepresentdatasuggesttheexistenceofanovelroleforTel2,namelyintranscription,possiblyin cooperationwithRvb2andinvolvingtheexistenceofphysicalinteractionswiththeMed15/Gal11Mediatorsubunit. Citation: Grandin N, Corset L, Charbonneau M (2012) Genetic and Physical Interactions between Tel2 and the Med15 Mediator Subunit in Saccharomyces cerevisiae.PLoSONE7(1):e30451.doi:10.1371/journal.pone.0030451 Editor:ArthurJ.Lustig,TulaneUniversityHealthSciencesCenter,UnitedStatesofAmerica ReceivedAugust16,2011;AcceptedDecember16,2011;PublishedJ