Genetic Variance in the Adiponutrin Gene Family and Childhood Obesity 英文参考文献.docVIP

GeneticVarianceintheAdiponutrinGeneFamilyand ChildhoodObesity LovisaE.Johansson1*,LinaM.Johansson1,PernillaDanielsson2,SvanteNorgren2,StinaJohansson2, ClaudeMarcus2,MartinRidderstra?le1 1Department ofClinical Sciences Malmo¨,LundUniversity, Lund, Sweden, 2NationalChildhoodObesityCenter, DivisionofPediatrics, Department ofClinical Science, Intervention,andTechnology,KarolinskaInstitutet,Stockholm,Sweden Abstract Aim:Theadiponutringenefamilyconsistsoffivegenes(PNPLA1-5)codingforproteinswithbothlipolyticandlipogenic properties. PNPLA3 has previously been associated with adult obesity. Here we investigated the possible association betweengeneticvariantsinthesegenesandchildhoodandadolescentobesity. Methods/Results:Polymorphismsinthefivegenesoftheadiponutringenefamilywereselectedandgenotypedusingthe Sequenomplatforminachildhoodandadolescentobesitycase-controlstudy.SixvariantsinPNPLA1showedassociation withobesity(rs9380559,rrs1467912,rs4713951,randrp,0.05afteradjustmentforage andgender).ThreevariantsinPNPLA3showedassociationwithobesitybefore,butnotafter,adjustmentforageandgender (rs139051, r and rs2072907, p.0.05). When analyzing these SNPs in relation to phenotypes, two SNPs in the PNPLA3 gene showed association with insulin sensitivity (r b=20.053, p=0.016, and rs2072907: b=20.049, p=0.024).NoassociationswereseenforPNPLA2,PNPLA4,andPNPLA5. Conclusions:Geneticvariationintheadiponutringenefamilydoesnotseemtocontributestronglytoobesityinchildren and adolescents. PNPLA1 exhibited a modest effect on obesity and PNPLA3 on insulin sensitivity. These data, however, requireconfirmationinothercohortsandethnicgroups. Citation: Johansson LE, Johansson LM, Danielsson P, Norgren S, Johansson S, et al. (2009) Genetic Variance in the Adiponutrin Gene Family and Childhood Obesity.PLoSONE4(4):e5327.doi:10.1371/journal.pone.0005327 Editor:AimeeK.Zaas,DukeUniversity,UnitedStatesofAmerica ReceivedAugust22,2008;AcceptedMarch26,2009;Publish