Genome-Wide Identification of Schistosoma japonicum MicroRNAs Using a Deep-Sequencing Approach 英文参考文献.docVIP

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Genome-Wide Identification of Schistosoma japonicum MicroRNAs Using a Deep-Sequencing Approach 英文参考文献.doc

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Genome-Wide Identification of Schistosoma japonicum MicroRNAs Using a Deep-Sequencing Approach 英文参考文献

Genome-WideIdentificationofSchistosomajaponicum MicroRNAsUsingaDeep-SequencingApproach JianHuang1,PeiHao2,HuiChen1,WeiHu3,QingYan1,FengLiu1,Ze-GuangHan1* 1Shanghai-MOSTKey Laboratory for Diseaseand Health Genomics, Chinese National Human Genome Centerat Shanghai, Shanghai, China, 2Shanghai Institutes for BiologicalSciences,ChineseAcademyofSciences,Shanghai,China,3NationalInstituteofParasiticDiseases,ChineseCenterforDiseaseControlandPrevention,Shanghai, China Abstract Background: Humanschistosomiasis is one of themost prevalent andseriousparasitic diseases worldwide. Schistosoma japonicumisoneofimportantpathogensofthisdisease.MicroRNAs(miRNAs)arealargegroupofnon-codingRNAsthat play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAsinagivenorganismisacriticalsteptofacilitatingourunderstandingofgenomeorganization,genomebiology, evolution,andposttranscriptionalregulation. Methodology/PrincipalFindings:WesequencedtwosmallRNAlibrariespreparedfromdifferentstagesofthelifecycleof S.japonicum,immatureschistosomulaandmaturepairingadults,throughadeepDNAsequencingapproach,whichyielded ,12 million high-quality short sequence reads containing a total of ,2 million non-redundant tags. Based on a bioinformatics pipeline, we identified 176 new S. japonicum miRNAs, of which some exhibited a differential pattern of expression between the two stages. Although 21 S. japonicum miRNAs are orthologs of known miRNAs within the metazoans,somenucleotidesatmanypositionsofSchistosomamiRNAs,suchasmiR-8,let-7,miR-10,miR-31,miR-92,miR- 124,andmiR-125,areindeedsignificantlydistinctfromotherbilaterianorthologs.Inaddition,bothmiR-71andsomemiR-2 familymembersintandemarefoundtobeclusteredinareversaldirectionmodelontwogenomicloci,andtwopairsof novelS.japonicummiRNAswerederivedfromsenseandantisenseDNAstrandsatthesamegenomicloci. Conclusions/Significance:ThecollectionofS.japonicummiRNAscouldbeusedasanewplatformtostudythegenomic structur