Molecular Characterization and Phylogenetic Study of Coxsackievirus A24v Causing Outbreaks of Acute Hemorrhagic Conjunctivitis (AHC) in Brazil 英文参考文献.docVIP

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Molecular Characterization and Phylogenetic Study of Coxsackievirus A24v Causing Outbreaks of Acute Hemorrhagic Conjunctivitis (AHC) in Brazil 英文参考文献.doc

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Molecular Characterization and Phylogenetic Study of Coxsackievirus A24v Causing Outbreaks of Acute Hemorrhagic Conjunctivitis (AHC) in Brazil 英文参考文献

MolecularCharacterizationandPhylogeneticStudyof CoxsackievirusA24vCausingOutbreaksofAcute HemorrhagicConjunctivitis(AHC)inBrazil FernandoNetoTavares1,RenatadeMendonc?a Campos1,FernandaMarcicanoBurlandy1 ,Rachel Fontella2,MariaMabelMontedeMelo3,ElianeVeigadaCosta1,EdsonEliasdaSilva1* 1Laborato′riodeEnterov?′rus,InstitutoOswaldoCruz/Fiocruz,RiodeJaneiro,RiodeJaneiro,Brazil,2Laborato′riodeBiologiaEvolutivaTeo′ricaeAplicada,Departamento deGene′tica,UniversidadeFederaldoRiodeJaneiro,RiodeJaneiro,RiodeJaneiro,Brazil,3Laborato′riodeVirologia-Laborato′rioCentraldeSau′dePu′blica(LACEN), Recife,Pernambuco,Brazil Abstract Background:CoxsackievirusA24variant(CA24v)isthemostprevalentviralpathogenassociatedwithacutehemorrhagic conjunctivitis(AHC)outbreaks.SixteenyearsafteritsfirstoutbreakinBrazil,thisagentreemergedin2003inBrazil,spreadto nearlyallstatesandcausedoutbreaksuntil2005.In2009,anewoutbreakoccurredinthenortheastregionofthecountry. In this study, we performed a viral isolation in cell culture and characterized clinical samples collected from patients presentingsymptomsduringtheoutbreakof2005inVito′ria,Esp?′ritoSantoState(ES)andtheoutbreakof2009inRecife, PernambucoState(PE).WealsoperformedaphylogeneticanalysisofworldwidestrainsandallmeaningfulBrazilianisolates since2003. MethodsandFindings:Sterilecottonswabswereusedtocollecteyedischarges,andall210clinicalsampleswereusedto inoculatecellcultures.CytopathiceffectsinHEp-2cellswereseenin58of180(32%)samplesfromVito′riaand3of30(10%) samplesfromRecife.PhylogeneticanalysisbasedonafragmentoftheVP1and3CgenerevealedthattheCA24vcausing outbreaksinBrazilduringtheyears2003,2004and2005evolvedfromAsianisolatesthathadcausedtheSouthKorean outbreakofAHCduringthesummerof2002.However,the2009outbreakofAHCinPernambucowasoriginatedfromthe reintroduction of a new CA24v strain that was circulating during 2007 in Asia, where CA24v outbreaks has been continuouslyreportedsince1970. Conclusions:ThisstudyisthefirstphylogeneticanalysisofAHCoutbreaksca