Monitoring Bacterial Community of Human Gut Microbiota Reveals an Increase in Lactobacillus in Obese Patients and Methanogens in Anorexic Patients 英文参考文献.docVIP

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Monitoring Bacterial Community of Human Gut Microbiota Reveals an Increase in Lactobacillus in Obese Patients and Methanogens in Anorexic Patients 英文参考文献.doc

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Monitoring Bacterial Community of Human Gut Microbiota Reveals an Increase in Lactobacillus in Obese Patients and Methanogens in Anorexic Patients 英文参考文献

MonitoringBacterialCommunityofHumanGut MicrobiotaRevealsanIncreaseinLactobacillusinObese PatientsandMethanogensinAnorexicPatients FabriceArmougom1,MireilleHenry1,BernardVialettes2,DenisRaccah3,DidierRaoult1* 1URMITE - UMR CNRS 6236, IRD 3R198, Universite′ de la Me′diterrane′e, Faculte′ de me′decine, Marseille, France, 2Service de Nutrition, Maladies Me′taboliques et Endocrinologie, UMR Universite′ me′diterrane′e-INRA U1260, CHU de la Timone, Marseille, France, 3Nutrition and Diabetology Department, University Hospital Sainte Marguerite,Marseille,France Abstract Background:StudiesofthebacterialcommunitiesofthegutmicrobiotahaverevealedashiftintheratioofFirmicutesand Bacteroidetes in obese patients. Determining the variations of microbial communities in feces may be beneficial for the identificationofspecificprofilesinpatientswithabnormalweights.TherolesofthearchaeonMethanobrevibactersmithii andLactobacillusspecieshavenotbeendescribedinthesestudies. Methods and Findings: Wedeveloped anefficient androbust real-time PCR tool that includes a plasmid-based internal control and allows for quantification of the bacterial divisions Bacteroidetes, Firmicutes, and Lactobacillus as well as the methanogenM.smithii.Weappliedthistechniquetothefecesof20obesesubjects,9patientswithanorexianervosa,and 20 normal-weight healthy controls. Our results confirmed a reduction in the Bacteroidetes community in obese patients (p,0.01). We found a significantly higher Lactobacillus species concentration in obese patients than in lean controls (p=0.0197)oranorexicpatients(p=0.0332).TheM.smithiiconcentrationwasmuchhigherinanorexicpatientsthaninthe leanpopulation(p=0.0171). Conclusions:Lactobacillusspeciesarewidelyusedasgrowthpromotersinthefarmindustryandarenowlinkedtoobesity in humans. The study of the bacterial flora in anorexic patients revealed an increase in M. smithii. This increase might representanadaptiveuseofnutrientsinthispopulation. Citation: Armougom F, Henry M, Vialettes B, Raccah D,