Next Generation Sequencing Provides Rapid Access to the Genome of Puccinia striiformis f. sp. tritici, the Causal Agent of Wheat Stripe Rust 英文参考文献.docVIP

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Next Generation Sequencing Provides Rapid Access to the Genome of Puccinia striiformis f. sp. tritici, the Causal Agent of Wheat Stripe Rust 英文参考文献.doc

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Next Generation Sequencing Provides Rapid Access to the Genome of Puccinia striiformis f. sp. tritici, the Causal Agent of Wheat Stripe Rust 英文参考文献

NextGenerationSequencingProvidesRapidAccessto theGenomeofPucciniastriiformisf.sp.tritici,theCausal AgentofWheatStripeRust DarioCantu1,ManjulaGovindarajulu2,AlexKozik2,MeinanWang3,XianmingChen3,4,KenjiK.Kojima5, JerzyJurka5,RichardW.Michelmore1,2,JorgeDubcovsky1,6,7 * 1DepartmentofPlantSciences,UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica,2GenomeCenter,UniversityofCaliforniaDavis,Davis,California, United States of America, 3Department of Plant Pathology, Washington State University, Pullman, Washington, United States of America, 4Wheat Genetics, Quality, Physiology,andDiseaseResearchUnit,UnitedStatesDepartmentofAgriculture-AgricultureResearchService(USDA-ARS),Pullman,Washington,UnitedStatesofAmerica, 5GeneticInformationResearchInstitute,MountainView,California,UnitedStatesofAmerica,6HowardHughesMedicalInstitute,ChevyChase,Maryland,UnitedStates ofAmerica,7GordonandBettyMooreFoundation,PaloAlto,California,UnitedStatesofAmerica Abstract Background:Thewheatstriperustfungus(Pucciniastriiformisf.sp.tritici,PST)isresponsibleforsignificantyieldlossesin wheat production worldwide. In spite of its economic importance, the PST genomic sequence is not currently available. Fortunately Next Generation Sequencing (NGS) has radically improved sequencing speed and efficiency with a great reduction in costs compared to traditional sequencing technologies. We used Illumina sequencing to rapidly access the genomicsequenceofthehighlyvirulentPSTrace130(PST-130). Methodology/PrincipalFindings:Weobtainednearly80millionhighqualitypaired-endreads(.50xcoverage)thatwere assembledinto29,178contigs(64.8Mb),whichprovideanestimatedcoverageofatleast88%ofthePSTgenesandare available through GenBank. Extensive micro-synteny with the Puccinia graminis f. sp. tritici (PGTG) genome and high sequence similarity with annotated PGTG genes support the quality of the PST-130 contigs. We characterized the transposableelementspresentinthePST-130contigsandusinganabinitiogenepredictionprogr