Observation of T-2 Toxin and HT-2 Toxin Glucosides from Fusarium sporotrichioides by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MSMS) 英文参考文献.docVIP

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Observation of T-2 Toxin and HT-2 Toxin Glucosides from Fusarium sporotrichioides by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MSMS) 英文参考文献.doc

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Observation of T-2 Toxin and HT-2 Toxin Glucosides from Fusarium sporotrichioides by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MSMS) 英文参考文献

Toxins 2011, 3, 1554-1568; doi:10.3390/toxins3121554 OPEN ACCESS toxins ISSN 2072-6651 /journal/toxins Article Observation of T-2 Toxin and HT-2 Toxin Glucosides from Fusarium sporotrichioides by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS) Mark Busman *, Stephen M. Poling and Chris M. Maragos U.S. Agricultural Research Service, National Center for Agricultural Utilization Department of Agriculture Research, Peoria, IL 61604, USA; E-Mails: Stephen.Poling@ (S.M.P.); Chris.Maragos@ (C.M.M.) * Author to whom correspondence should be addressed; E-Mail: Mark.Busman@; Tel.: +1-309-681-6241; Fax: +1-309-681-6293. Received: 24 November 2011; in revised form: 14 December 2011 / Accepted: 19 December 2011 / Published: 20 December 2011 Abstract: The trichothecenes produced by solid and liquid cultures of Fusarium sporotrichioides were evaluated with high performance liquid chromatography—tandem mass spectrometry (LC-MS/MS). Along with the expected T-2 toxin HT-2 toxin and neosolaniol, two additional compounds were detected, which had ions 162 m/z higher than those in the mass spectra of T-2 toxin or HT-2 toxin. Fragmentation behavior of these two compounds was similar to that of T-2 toxin and HT-2 toxin. Based on LC-MS/MS behavior, it is proposed that the two compounds are T-2 toxin 3-O-glucoside and HT-2 toxin 3-O-glucoside. Production of the two glucosides was measured in kernels from wheat and oat inoculated with F. sporotrichiodes, as well as in cultures grown in liquid media and on cracked corn or rice. Production of glucosides in wheat and oats suggest that they may also be present in naturally contaminated cereals. Keywords: Fusarium sporotrichioides; glucoside; trichothecene; wheat; oats; mass spectrometry Toxins 2011, 3 1555 1. Introduction T-2 toxin (Figure 1a) is one of a group of trichothecene mycotoxins produced by various species of Fusaria, includi