Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen 英文参考文献.docVIP

下载本文档

10
0
约4.72万字
约 9页
2017-05-12 发布于上海
举报
版权申诉

Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen AbdelMounim Hamman-Khalifa, Antonio Jesús Castro, José Carlos Jiménez- López, María Isabel Rodríguez-García and Juan de Dios Alché* Address: Department of Biochemistry, Cell and Molecular Biology of Plants, Estación, Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain Email: AbdelMounim Hamman-Khalifa - abdelmounin@ugr.es; Antonio Jesús Castro - antoniojesus.castro@eez.csic.es; José Carlos Jiménez- López - josecarlos.jimenez@eez.csic.es; María Isabel Rodríguez-García - mariaisabel.rodriguez@eez.csic.es; Juan de Dios Alché* - juandedios.alche@eez.csic.es * Corresponding author Published: 25 January 2008 Received: 18 July 2007 Accepted: 25 January 2008 BMC Plant Biology 2008, 8:10 doi:10.1186/1471-2229-8-10 This article is available from: /1471-2229/8/10 ? 2008 Hamman-Khalifa et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Pollens from different olive (Olea europaea L.) cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is, in addition, characterized by a high degree of polymorphism in its sequence. The purpose of this study is to evaluate the putative presence of divergences in Ole e 1 sequences from different olive cultivars. Results: RNA from pollen individually collected from 10 olive cultivars was used to amplify Ole e 1 sequences by RT-PCR, and the sequences were analyzed by using different bioinformatics tools. Numerous nucleotide substitutions were detected throughout the sequences