Optimization of technology for dietary fiber extraction from Maixiansan by response surface methodology 英文参考文献.docVIP

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Optimization of technology for dietary fiber extraction from Maixiansan by response surface methodology 英文参考文献.doc

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Optimization of technology for dietary fiber extraction from Maixiansan by response surface methodology 英文参考文献

Lvetal.ChineseMedicine2012,7:28 /content/7/1/28 RESEARCH OpenAccess Optimizationoftechnologyfordietaryfiber extractionfromMaixiansanbyresponsesurface methodology Hui-qingLv1,ChangfengHu2,Hai-pingZhong1,Hong-binZheng1*andChengpingWen2* Abstract Background:Ourstudyaimstodeterminewhetherresponsesurfacemethodologycanoptimizetheextractionof dietaryfiberfromMaixiansan. Methods:ABox–Behnkendesignwasemployedtooptimizetheextractionparameters,includingα-amylase concentration(X1:0.3–0.5%),enzymolysistime(X2:30–60min)andNaOHcontent(X3:1.0–5.0%),ofdietaryfiber fromMaixiansanusinganenzyme–alkaliextractiontechnique. Results:Theoptimaltechnologicalconditionswereasfollows:α-amylaseconcentration:0.4%;enzymolysistime:45 min;NaOHcontent:4.0%.Undertheseconditions,theextractionyieldreached57.14%,whichwaswellconsistent withthepredictedmodelswithacoefficientofdetermination(R2)of0.9818.Anevaluationoftheanti-inflammatory activityindicatedthatMaixiansanwasabletosignificantlyinhibitdextransodiumsulfate-inducedulcerativecolitis inratsbyincreasingtheconcentrationofshort-chainfattyacids(acetate,propionateandbutyrate),amongwhich thebutyratecontentwassignificantlyhigherintheMaixiansangroupthanintheothergroups. Conclusion:Ourexperimentsshowedthatresponsesurfacemethodologycanoptimizetheextractionofdietary fiberfromMaixiansan.Maixiansancouldbeexploredasananti-ulcerativecolitisagent. Background diarrheaandmucosaldamage,andexertssignificanteffects Inflammatory bowel disease includes Crohn’s disease and onUC[7]. ulcerative colitis (UC). UC is a chronic inflammatory The common approach of an orthogonal test design is disorder of the colon characterized by alternating periods usually adopted to optimize the extraction conditions for of flare-ups and quiescent disease [1]. Dietary fiber is an dietary fiber from Maixiansan (DFM) to achieve higher integral constituent of normal human nutrition and may yieldandquality,buttheeffectsofinteractionsbetweendif- contribute to a h