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OryzaPG-DB Rice Proteome Database based on Shotgun Proteogenomics 英文参考文献
Helmyetal.BMCPlantBiology2011,11:63
/1471-2229/11/63
DATABASE
OpenAccess
OryzaPG-DB:RiceProteomeDatabasebasedon
ShotgunProteogenomics
MohamedHelmy1,2,MasaruTomita1andYasushiIshihama1,3*
Abstract
Background:Proteogenomicsaimstoutilizeexperimentalproteomeinformationforrefinementofgenome
annotation.Sincemassspectrometry-basedshotgunproteomicsapproachesprovidelarge-scalepeptide
sequencingdatawithhighthroughput,adatarepositoryforshotgunproteogenomicswouldrepresentavaluable
sourceofgeneexpressionevidenceatthetranslationallevelforgenomere-annotation.
Description:Here,wepresentOryzaPG-DB,ariceproteomedatabasebasedonshotgunproteogenomics,which
incorporatesthegenomicfeaturesofexperimentalshotgunproteomicsdata.Thisversionofthedatabasewas
createdfromtheresultsof27nanoLC-MS/MSrunsonahybridiontrap-orbitrapmassspectrometer,whichoffers
highaccuracyforanalyzingtrypticdigestsfromundifferentiatedculturedricecells.Peptideswereidentifiedby
searchingtheproductionspectraagainsttheprotein,cDNA,transcriptandgenomedatabasesfromMichigan
StateUniversity,andweremappedtothericegenome.Approximately3200geneswerecoveredbythese
peptidesand40ofthemcontainednovelgenomicfeatures.Userscansearch,downloadornavigatethedatabase
perchromosome,gene,protein,cDNAortranscriptanddownloadtheupdatedannotationsinstandardGFF3
format,withvisualizationinPNGformat.Inaddition,thedatabaseschemeofOryzaPGwasdesignedtobegeneric
andcanbereusedtohostsimilarproteogenomicinformationforotherspecies.OryzaPGisthefirst
proteogenomics-baseddatabaseofthericeproteome,providingpeptide-basedexpressionprofiles,togetherwith
thecorrespondinggenomicorigin,includingtheannotationofnoveltyforeachpeptide.
Conclusions:TheOryzaPGdatabasewasconstructedandisfreelyavailableathttp://oryzapg.iab.keio.ac.jp/.
Background
annotation is highly dependent on the expression evi-
Amonghigh-throughputexperimentalmethods,genome dence, usually transcriptional, provided byexperiments
sequencing represents a turning point in the under- and the algorithms impl
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