Oscillatory Dynamics of Cell Cycle Proteins in Single Yeast Cells Analyzed by Imaging Cytometry 英文参考文献.docVIP

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Oscillatory Dynamics of Cell Cycle Proteins in Single Yeast Cells Analyzed by Imaging Cytometry 英文参考文献.doc

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Oscillatory Dynamics of Cell Cycle Proteins in Single Yeast Cells Analyzed by Imaging Cytometry 英文参考文献

OscillatoryDynamicsofCellCycleProteinsinSingle YeastCellsAnalyzedbyImagingCytometry DavidA.Ball1,JulieMarchand1,MagalyPoulet1,WilliamT.Baumann2,KatherineC.Chen3,JohnJ. Tyson3,JeanPeccoud1,4* 1Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, Virginia, United States of America, 2Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg,Virginia,UnitedStatesofAmerica,3DepartmentofBiologicalSciences,VirginiaTech,Blacksburg,Virginia,UnitedStatesofAmerica,4InstituteforCritical TechnologyandAppliedScienceCenterforSystemsBiologyofEngineeredTissues,VirginiaTech,Blacksburg,Virginia,UnitedStatesofAmerica Abstract Progressionthroughthecelldivisioncycleisorchestratedbyacomplexnetworkofinteractinggenesandproteins.Someof theseproteinsareknowntofluctuateperiodicallyduringthecellcycle,butasystematicstudyofthefluctuationsofabroad sampleofcell-cycleproteinshasnotbeenmadeuntilnow.Usingtime-lapsefluorescencemicroscopy,weprofiled16strains ofbuddingyeast,eachcontainingGFPfusedtoasinglegeneinvolved incellcycle regulation.Thedynamics ofprotein abundanceandlocalizationwerecharacterizedbyextractingtheamplitude,period,andotherindicatorsfromaseriesof images. Oscillations of protein abundance could clearly be identified for Cdc15, Clb2, Cln1, Cln2, Mcm1, Net1, Sic1, and Whi5.Theperiodofoscillationofthefluorescentlytaggedproteinsisgenerallyingoodagreementwiththeinter-budtime. The very strong oscillations of Net1 and Mcm1 expression are remarkable since little is known about the temporal expressionofthesegenes.Bycollectingdatafromlargesamplesofsinglecells,wequantifiedsomeaspectsofcell-to-cell variabilityduepresumablytointrinsicandextrinsicnoiseaffectingthecellcycle. Citation:BallDA,MarchandJ,PouletM,BaumannWT,ChenKC,etal.(2011)OscillatoryDynamicsofCellCycleProteinsinSingleYeastCellsAnalyzedbyImaging Cytometry.PLoSONE6(10):e26272.doi:10.1371/journal.pone.0026272 Editor:DanielLew,DukeUniversityMedicalCenter,UnitedStatesofAmerica ReceivedFebruary12,2011;Accep