Oryza sativa Cytochrome P450 Family Member OsCYP96B4 Reduces Plant Height in a Transcript Dosage Dependent Manner 英文参考文献.docVIP

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Oryza sativa Cytochrome P450 Family Member OsCYP96B4 Reduces Plant Height in a Transcript Dosage Dependent Manner 英文参考文献.doc

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Oryza sativa Cytochrome P450 Family Member OsCYP96B4 Reduces Plant Height in a Transcript Dosage Dependent Manner 英文参考文献

OryzasativaCytochromeP450FamilyMember OsCYP96B4ReducesPlantHeightinaTranscriptDosage DependentManner RengasamyRamamoorthy¤,Shu-YeJiang*,SrinivasanRamachandran* RiceFunctionalGenomicsGroup,TemasekLifeSciencesLaboratory,NationalUniversityofSingapore,Singapore,Singapore Abstract Background:PlantcytochromesP450areinvolvedinawiderangeofbiosyntheticreactionsandplayvariousrolesinplant development.However,littleisknownaboutthebiologicalfunctionsofthesubfamilyCYP96inplants. Methodology/Principal Findings:Here, wereportanovelsemi-dwarfricemutant,inwhicha singlecopy oftransposon dissociator(Ds)wasinsertedintothegeneOsCYP96B4(OryzasativaCytochromeP45096B4).Themutantexhibitsthedefects incellelongationandpollengermination,whichcanbecomplementedbythewildtypeOsCYP96B4andberescuedby remobilization of the Ds element with the presence of the transposase Activator (Ac). Transgenic plants harboring OsCYP96B4 double-stranded RNA interference construct mimicked the mutant phenotype. The oscyp96b4 mutant phenotypecouldnotberescuedbyallthetestedphytohormonesanditwasfoundthatOsCYP96B4reducedplantheightin atranscriptdosagedependentmanner.HeterologousexpressionofOsCYP96B4inSchizosaccharomycespomberesultedin missegregationandwidercells.Furtherinvestigationshowedthatthemutantexhibitedthedefectsinthemetabolismof somelipidmolecularspecieswhencomparedwiththewildtype. Conclusions/Significance: The oscyp96b4 mutant is a novel rice semi-dwarf mutant. Our data suggest that OsCYP96B4 mightbeinvolvedinlipidmetabolismandregulatecellelongation. Citation:RamamoorthyR,JiangS-Y,RamachandranS(2011)OryzasativaCytochromeP450FamilyMemberOsCYP96B4ReducesPlantHeightinaTranscript DosageDependentManner.PLoSONE6(11):e28069.doi:10.1371/journal.pone.0028069 Editor:JuergenKroymann,FrenchNationalCentreforScientificResearch,Universite′ Paris-Sud,France ReceivedMay12,2011;AcceptedOctober31,2011;PublishedNovember28,2011 Copyright:?2011Ramamoorthyetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreat