Pb-induced cellular defense system in the root meristematic cells of Allium sativum L 英文参考文献.docVIP
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Pb-induced cellular defense system in the root meristematic cells of Allium sativum L 英文参考文献
JiangandLiuBMCPlantBiology2010,10:40
/1471-2229/10/40
RESEARCH ARTICLE
OpenAccess
Pb-inducedcellulardefensesystemintheroot
meristematiccellsofAlliumsativumL
WushengJiang1,DonghuaLiu2*
Abstract
Background:Electronmicroscopy(EM)techniquesenableidentificationofthemainaccumulationsoflead(Pb)in
cellsandcellularorganellesandobservationsofchangesincellultrastructure.Althoughthereisextensiveliterature
relatingtostudiesontheinfluenceofheavymetalsonplants,Pbtolerancestrategiesofplantshavenotyetbeen
fullyexplained.AlliumsativumL.isapotentialplantforabsorptionandaccumulationofheavymetals.Inprevious
investigationstheeffectsofdifferentconcentrations(10-5 to10-3 M)ofPbwereinvestigatedinA.sativum,
indicatingasignificantinhibitoryeffectonshootandrootgrowthat10-3 to10-4 MPb.Inthepresentstudy,we
usedEMandcytochemistrytoinvestigateultrastructuralalterations,identifythesynthesisanddistributionof
cysteine-richproteinsinducedbyPbandexplainthepossiblemechanismsofthePb-inducedcellulardefense
systeminA.sativum.
Results:After1hofPbtreatment,dictyosomeswereaccompaniedbynumerousvesicleswithincytoplasm.The
endoplasmreticulum(ER)withswollencisternaewasarrangedalongthecellwallafter2h.Someflattened
cisternaewerebrokenupintosmallclosedvesiclesandthenuclearenvelopewasgenerallymoredilatedafter4h.
During24-36h,phenomenaappearedsuchashighvacuolizationofcytoplasmandelectron-densegranulesincell
walls,vacuoles,cytoplasmandmitochondrialmembranes.Otherchangesincludedmitochondrialswellingandloss
ofcristae,andvacuolizationofERanddictyosomesduring48-72h.InthePb-treatmentgroups,silvergrainswere
observedincellwallsandincytoplasm,suggestingtheGomori-SwiftreactioncanindirectlyevaluatethePbeffects
onplantcells.
Conclusions:CellwallscanimmobilizesomePbions.Cysteine-richproteinsincellwallswereconfirmedbythe
Gomori-Swiftreaction.Themorphologicalalterationsinplasmamembrane,dictyosomesandERreflectthefeatures
ofdetoxificationandtoleranceunderPbstress.VacuolesareultimatelyoneofmainstoragesitesofPb.Root
meristem
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