Pericyte-Like Progenitors Show High Immaturity and Engraftment Potential as Compared with Mesenchymal Stem Cells 英文参考文献.docVIP

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Pericyte-Like Progenitors Show High Immaturity and Engraftment Potential as Compared with Mesenchymal Stem Cells 英文参考文献.doc

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Pericyte-Like Progenitors Show High Immaturity and Engraftment Potential as Compared with Mesenchymal Stem Cells 英文参考文献

Pericyte-LikeProgenitorsShowHighImmaturityand EngraftmentPotentialasComparedwithMesenchymal StemCells AminaBouacida1,2,PhilippeRosset3,4,Vale′rieTrichet3,FabienGuilloton1,NicolasEspagnolle1, ThomasCordonier3,DominiqueHeymann3,PierreLayrolle3,LucSense′be′1,Fre′de′ricDeschaseaux1* 1StromalabUnite′ MixtedeRecherche(UMR)Universite′ PaulSabatier/CentreNationaldelaRechercheScientifique(CNRS)5273,U1031Institutnationaldelasante′ etde larechercheme′dicale(Inserm),EtablissementFranc?aisduSang-Pyre′ne′es-Me′diterrane′e, Toulouse,France,2EA3855,Universite′ Franc?ois Rabelais,Tours,France,3Inserm UMR957,PhysiopathologiedelaRe′sorptionOsseuseetThe′rapiedesTumeursOsseusesPrimitives,Universite′deNantes,Nantes,France,4CentreHospitalierUniversitaire Trousseau,Tours,France Abstract Mesenchymal stem cells (MSCs) and pericyte progenitors (PPs) are both perivascular cells with similar multipotential properties regardless of tissueof origin.We comparedthephenotype andfunctionof the2cell types derivedfrom the samebone-marrowsamplesbutexpandedintheirrespectivemedia–pericyteconditions(endothelialcellgrowthmedium 2 [EGM-2]) for PPs and standard medium (mesenchymal stem cell medium [MSM]) for MSCs. After 3 weeks of culture, whatever the expansion medium, all cells showed similar characteristics (MSC markers and adipo-osteo-chondroblastic differentiationpotential),althoughneuronalpotentialwasgreaterinEGM-2–thanMSM-culturedcells.Ascomparedwith MSM-cultured MSCs, EGM-2–cultured PPs showed higher expression of the pericyte-specific antigen 3G5 than a-smooth muscleactin.Inaddition,EGM-2–culturedPPsshowedanimmaturephenotype,withupregulationofstemnessOCT4and SOX2 proteins and downregulation of markers of osteoblastic, chondroblastic, adipocytic and vascular smooth muscle lineages.DespitehavinglesseffectiveinvitroimmunosuppressioncapacitiesthanstandardMSCs,EGM-2–culturedPPshad higherengraftmentpotentialswhencombinedwithbiomaterialsheterotopically-transplantedinNudemice.Furthermore, the