Potential for Development of an Escherichia coli—Based Biosensor for Assessing Bioavailable Methionine A Review 英文参考文献.docVIP

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Potential for Development of an Escherichia coli—Based Biosensor for Assessing Bioavailable Methionine A Review 英文参考文献.doc

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Potential for Development of an Escherichia coli—Based Biosensor for Assessing Bioavailable Methionine A Review 英文参考文献

Sensors 2010, 10, 3562-3584; doi:10.3390/s100403562 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Potential for Development of an Escherichia coli—Based Biosensor for Assessing Bioavailable Methionine: A Review Vesela I. Chalova 1,2,?, Clifford A. Froelich, Jr. 1,? and Steven C. Ricke 1,2, * 1 Poultry Science Department, Texas AM University, College Station, TX 77843-2472, USA; E-Mails: veselachalova@ (V.I.C.); CFroel@ (C.A.F.) Center for Food Safety and Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA 2 ? ? Current address: Department of Biochemistry and Molecular Biology, University of Food Technologies, Plovdiv, Bulgaria. Current address: Department of Biochemistry and Molecular Biology, Louisiana State University Health Science Center, Shreveport, LA 71129, USA. * Author to whom correspondence should be addressed; E-Mail: sricke@; Tel.: +1-(479) 575-4678; Fax: +1-(479) 575-6936. Received: 8 February 2010; in revised form: 11 March 2010 / Accepted: 26 March 2010 / Published: 8 April 2010 Abstract: Methionine is an essential amino acid for animals and is typically considered one of the first limiting amino acids in animal feed formulations. Methionine deficiency or excess in animal diets can lead to sub-optimal animal performance and increased environmental pollution, which necessitates its accurate quantification and proper dosage in animal rations. Animal bioassays are the current industry standard to quantify methionine bioavailability. However, animal-based assays are not only time consuming, but expensive and are becoming more scrutinized by governmental regulations. In addition, a variety of artifacts can hinder the variability and time efficacy of these assays. Microbiological assays, which are based on a microbial response to external supplementation of a particular nutrient such as methionine, appear to be attractive potential alternatives to the alre