Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots 英文参考文献.docVIP

Molecules 2012, 17, 1635-1651; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots Aida Firdaus Muhammad Nurul Azmi 1,2, Shuhaimi Mustafa 3,*, Dzulkifly Md. Hashim 3,4 and Yazid Abdul Manap 4 1 Department of Microbiology, Faculty Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia 2 Department of Food Technology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia 3 Halal Products Research Institute, Universiti Putra Malaysia, Putra Infoport, 43400 Serdang Selangor, Malaysia 4 Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia * Author to whom correspondence should be addressed; E-Mail: shuhaimi@.my. Received: 11 October 2011; in revised form: 28 December 2011 / Accepted: 13 January 2012 / Published: 7 February 2012 Abstract: Bamboo shoot crude polysaccharides (BSCP) extracted from the shoots of Gigantochloa levis gave about 3.27 ± 0.18% on dry basis and a very minute percentage of protein (0.02 ± 0.01%). The molecular weight of BSCP estimated by gel chromatography was found to be around 7.49 × 103 Da, while the molecular weights of purified fractions (F1 to F5) were around 1550.96, 1471.63, 1685.78, 1691.61 and 1551.67 Da, respectively. The FTIR spectrum of BSCP revealed the possibility that the extract contains?β-glucan, which can be considered a valuable compound for the medical and food industries. These relate to the resistance of BSCP towards artificial human gastric juice which is more than 99%. Prebiotic activity tested using BSCP as a carbon source showed significant increase in the growth of B. animalis ATCC 1053, B. longum BB 536 and L. acidophilus ATCC 4356 as compared to t