Process development for the elucidation of mycotoxin formation in Alternaria alternata 英文参考文献.docVIP
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Process development for the elucidation of mycotoxin formation in Alternaria alternata 英文参考文献
Brzonkaliketal.AMBExpress2011,1:27
/content/1/1/27
ORIGINAL
OpenAccess
Processdevelopmentfortheelucidationof
mycotoxinformationinAlternariaalternata
KatrinBrzonkalik*,TanjaHerrling,ChristophSyldatkandAnkeNeumann
Abstract
TheblackmouldAlternariaalternataproducesawidediversityofmycotoxinswhichareofparticularhealth
concern.SincenomaximumallowablelimitsaresetforAlternariatoxinsinfoodandfeed,preventionofAlternaria
infestationsandmycotoxinspoilageistheonlywaytoavoidhealthrisks.Thus,theunderstandingofmycotoxin
biosynthesisisessential.Forthatpurpose,areliablebatchprocessina2Lbioreactorwasestablishedwhich
enablesthestudyofseveralparametersinfluencingtheproductionofthemycotoxinsalternariol(AOH),alternariol
monomethylether(AME)andtenuazonicacid(TA)byA.alternataDSM12633.ModifiedCzapek-Doxmediumwas
usedwithglucoseascarbonsourceandammoniumandnitrateasnitrogensources.Consumptionofcarbonand
nitrogensourcesaswellasformationofthethreemycotoxinsweremonitored;theaveragedataoffive
independentfermentationswasplottedandfittedusingalogisticequationwithfourparameters.Maximum
mycotoxinconcentrationsof3.49±0.12mg/LAOH,1.62±0.14mg/LAMEand38.28±0.1mg/LTAwere
obtained.
Inthissystemtheeffectofdifferentaerationrates(0.53vvm-0.013vvm)wastestedwhichexertedagreat
influenceonmycotoxinproduction.Theuseofthesemi-syntheticCzapek-Doxmediumallowedtheexchangeof
carbonandnitrogensourcesforacetateandasparticacid.Theuseofacetateinsteadofglucoseresultedinthe
soleproductionofalternariolwhereastheexchangeofammoniumandnitrateforaspartateenhancedthe
productionofbothAOHandAMEwhileTAproductionwasnotaffected.
Keywords:Alternariaalternata,Mycotoxin,Batchprocess,Aerationrate
Introduction
possible.Theyareconnectedtocertainhealthdisorders
Mycotoxinsaresecondarymetabolitesoflowmolecular and elicit acute toxic, mutagenic, teratogenic, carcino-
weightproducedbyfilamentousfungi.Sincethediscov- genicandsometimesestrogenicproperties(Bhatnagaret
eryofthefirstmycotoxins,theaflatoxins,in1960which al.2002).Basedon
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