Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species 英文参考文献.docVIP
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Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species 英文参考文献
Molecules 2012, 17, 3093-3113; doi:10.3390/moleculeOPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Profiling of Phenolic Compounds and Antioxidant Activity of
Dry Extracts from the Selected Sorbus Species
Monika A. Olszewska *, Anna Presler and Piotr Michel
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1,
90-151 Lodz, Poland; E-Mails: anpre@wp.pl (A.P.); piotr.michel@umed.lodz.pl (P.M.)
* Author to whom correspondence should be addressed; E-Mail: monika.olszewska@umed.lodz.pl;
Tel.: +48-42-677-9169; Fax: +48-42-678-8398.
Received: 15 February 2012; in revised form: 5 March 2012 / Accepted: 6 March 2012 /
Published: 12 March 2012
Abstract: The antioxidant efficiency of dry extracts from inflorescences and/or leaves of
seven Sorbus species was studied using four in vitro tests of SET (single electron transfer)
and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its
diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the
phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and Trolox?. The SET-type
activity of the extracts depended primarily on the extraction solvent. The most valuable
extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH
(EC50 = 3.2–5.2 μg/mL), TEAC (2.8–4.0 mmol Trolox?/g), and FRAP (9.8–13.7 mmol
Fe2+/g) tests, and strongly correlated with the total phenolic levels (39.6–58.2% of gallic
acid equivalents). The HPLC-PDA analysis of the extracts led to the identification of
chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and
sexangularetin 3-O-?-D-glucopyranoside as the main components. Apart from flavonoids
and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the
SET-type activity. The HAT-reactivity of the extracts in the l
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