Prokayrotic Ubiquitin-Like Protein (Pup) Proteome of Mycobacterium tuberculosis 英文参考文献.docVIP

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Prokayrotic Ubiquitin-Like Protein (Pup) Proteome of Mycobacterium tuberculosis 英文参考文献.doc

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Prokayrotic Ubiquitin-Like Protein (Pup) Proteome of Mycobacterium tuberculosis 英文参考文献

ProkayroticUbiquitin-LikeProtein(Pup)Proteomeof Mycobacteriumtuberculosis RichardA.Festa1.,FionaMcAllister2.,MichaelJ.Pearce1.,JulianMintseris2,KristinE.Burns1,StevenP. Gygi2*,K.HeranDarwin1* 1DepartmentofMicrobiology,NewYorkUniversitySchoolofMedicine,NewYork,NewYork,UnitedStatesofAmerica,2DepartmentofCellBiology,HarvardMedical School,Boston,Massachusetts,UnitedStatesofAmerica Abstract Prokaryotic ubiquitin-like protein (Pup) in Mycobacterium tuberculosis (Mtb) is the first known post-translational small proteinmodifierinprokaryotes,andtargetsseveralproteinsfordegradationbyabacterialproteasomeinamannerakinto ubiquitin(Ub)mediatedproteolysisineukaryotes.TodeterminetheextentofpupylationinMtb,weusedtandemaffinity purification to identify its ‘‘pupylome’’. Mass spectrometry identified 55 out of 604 purified proteins with confirmed pupylationsites.Forty-fourproteins,includingthosewithandwithoutidentifiedpupylationsites,weretestedassubstrates ofproteolysisinMtb.Understeadystateconditions,themajorityofthetestproteinsdidnotaccumulateindegradation mutants, suggesting not all targets of pupylation are necessarily substrates of the proteasome under steady state conditions.FourproteinsimplicatedinMtbpathogenesis,Icl(isocitratelyase),Ino1(inositol-1-phosphatesynthase),MtrA (MtbresponseregulatorA)andPhoP(phosphateresponseregulatorP),showedalteredlevelsindegradationdefectiveMtb. Icl, Ino1andMtrAaccumulatedin Mtbdegradation mutants,suggestingthese proteinsaretargetedtotheproteasome. Unexpectedly,PhoPwaspresentinwildtypeMtbbutundetectableinthedegradationmutants.Takentogether,thesedata demonstratethatpupylationregulatesnumerousproteinsinMtbandmaynotalwaysleadtodegradation. Citation: Festa RA, McAllister F, Pearce MJ, Mintseris J, Burns KE, et al. (2010) Prokayrotic Ubiquitin-Like Protein (Pup) Proteome of Mycobacterium tuberculosis.PLoSONE5(1):e8589.doi:10.1371/journal.pone.0008589 Editor:AnilKumarTyagi,UniversityofDelhi,India ReceivedNovember17,2009;AcceptedDecember9,2009;Publi